Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-GAL4 抗体 [EPR12710(B)] (ab175185)

Submit a review Submit a question
Western blot - Anti-GAL4 antibody [EPR12710(B)] (ab175185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)
  • Anti-GAL4 antibody [EPR12710(B)] (ab175185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12710(B)] to GAL4
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GAL4 antibody [EPR12710(B)]
    GAL4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12710(B)] to GAL4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Rat, Human
    非交差種: Mouse

  • 免疫原

    Synthetic peptide within Human GAL4 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P56470

  • ポジティブ･コントロール

    • HT-29, Human small intestine and rat small intestine lysates. Human colon and colon carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab175185の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
特記事項
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

  • 配列類似性

    Contains 2 galectin domains.

  • ドメイン

    Contains two homologous but distinct carbohydrate-binding domains.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antigen NY CO 27 antibody
      • Antigen NY-CO-27 antibody
      • Antigen NYCO27 antibody
      • GAL 4 antibody
      • Gal-4 antibody
      • GAL4 antibody
      • Galectin 4 antibody
      • Galectin-4 antibody
      • Galectin4 antibody
      • Homo sapiens galectin4 mRNA complete cds antibody
      • L 36 lactose binding protein antibody
      • L-36 lactose-binding protein antibody
      • L36 lactose binding protein antibody
      • L36LBP antibody
      • Lactose binding lectin 4 antibody
      • Lactose-binding lectin 4 antibody
      • Lectin galactoside binding soluble 4 antibody
      • LEG4_HUMAN antibody
      • LGALS4 antibody
      see all

    画像

    • Western blot - Anti-GAL4 antibody [EPR12710(B)] (ab175185)
      Western blot - Anti-GAL4 antibody [EPR12710(B)] (ab175185)
      All lanes : Anti-GAL4 antibody [EPR12710(B)] (ab175185) at 1/1000 dilution

      Lane 1 : HT-29 cell lysates
      Lane 2 : Human small intestine lysates
      Lane 3 : Rat small intestine lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 36 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)

      Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GAL4 with ab175185 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GAL4 with ab175185 at a 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-GAL4 antibody [EPR12710(B)] (ab175185)
      Anti-GAL4 antibody [EPR12710(B)] (ab175185)

    参考文献 (0)

    ab175185 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab175185 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab175185.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com