Anti-GADD34 抗体 (ab9869)
Key features and details
- Goat polyclonal to GADD34
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GADD34 antibody
GADD34 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GADD34 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human GADD34 aa 650 to the C-terminus (C terminal) (Cysteine residue). NP_055145.3.
Database link: O75807-1 -
ポジティブ･コントロール
- WB: HepG2 cell lysate. IHC-P: Human liver tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9869の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. -
配列類似性
Belongs to the PPP1R15 family. -
翻訳後修飾
Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. -
細胞内局在
Endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 23645 Human
- Entrez Gene: 17872 Mouse
- Omim: 611048 Human
- SwissProt: O75807 Human
- SwissProt: P17564 Mouse
- Unigene: 631593 Human
- Unigene: 4048 Mouse
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別名
- Apoptosis associated protein antibody
- GADD 34 antibody
- Growth arrest and DNA damage inducible 34 antibody
see all
画像
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Western blot - Anti-GADD34 antibody (ab9869)Anti-GADD34 antibody (ab9869) at 0.1 µg/ml + HEPG2 cell lysate at 35 µg
Predicted band size: 75 kDa
Primary incubation for 1 hour. Detection by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (ab9869)IHC image of ab9869 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9869, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (ab9869)ab9869 staining GADD34 in human liver sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab9869 は 10 報の論文で使用されています。
- Li X et al. Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2a/ATF4/CHOP pathway in breast cancer. Cell Death Dis 12:314 (2021). PubMed: 33762577
- Yuan T et al. miR-195 promotes LPS-mediated intestinal epithelial cell apoptosis via targeting SIRT1/eIF2a. Int J Mol Med 45:510-518 (2020). PubMed: 31894250
- Hou X et al. Galanthamine improves myocardial ischemia-reperfusion-induced cardiac dysfunction, endoplasmic reticulum stress-related apoptosis, and myocardial fibrosis by suppressing AMPK/Nrf2 pathway in rats. Ann Transl Med 7:634 (2019). PubMed: 31930035
- Li YH et al. Halofuginone protects against advanced glycation end products-induced injury of H9C2 cells via alleviating endoplasmic reticulum stress-associated apoptosis and inducing autophagy. Mol Med Rep 20:3131-3139 (2019). PubMed: 31432112
- Bahar E et al. Polyphenolic Extract of Euphorbia supina Attenuates Manganese-Induced Neurotoxicity by Enhancing Antioxidant Activity through Regulation of ER Stress and ER Stress-Mediated Apoptosis. Int J Mol Sci 18:N/A (2017). PubMed: 28146110
- Galindo I et al. The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection. Cell Death Dis 3:e341 (2012). WB . PubMed: 22764100
- Garrido JL et al. EBNA3C interacts with Gadd34 and counteracts the unfolded protein response. Virol J 6:231 (2009). PubMed: 20040105
- Kepp O et al. Disruption of the PP1/GADD34 complex induces calreticulin exposure. Cell Cycle 8:3971-7 (2009). PubMed: 19901557
- Panaretakis T et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J 28:578-90 (2009). WB ; Mouse . PubMed: 19165151
- Criollo A et al. Regulation of autophagy by the inositol trisphosphate receptor. Cell Death Differ 14:1029-39 (2007). WB ; Human . PubMed: 17256008
