AB175355

Anti-GADD34 抗体

Name: Anti-GADD34 抗体 (ab175355) | Abcam
Brand: Abcam Limited
SKU: AB175355
Price: 76000 JPY
Availability: InStock

Anti-GADD34 antibody

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(2 Publications)

Rabbit Polyclonal GADD34 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R15A aa 550-650.

別名を表示する

GADD34, PPP1R15A, Protein phosphatase 1 regulatory subunit 15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (AB175355)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (AB175355)

Immunofluorescent analysis of formalin-fixed HeLa cells labeling GADD34 with ab175355 at 1/50 and incubated with DyLight 488 Goat anti-rabbit IgG secondary antibody. F-actin (red) was stained with DyLight 650 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Supplier Data

Western blot - Anti-GADD34 antibody (AB175355)

Lane 2:

Western blot - Anti-GADD34 antibody (ab175355) at 1/250 dilution

Lanes 3 and 5:

Western blot - Anti-GADD34 antibody (ab175355) at 1/500 dilution

Lane 4:

Western blot - Anti-GADD34 antibody (ab175355) at 1/1000 dilution

Lanes 2 - 4:

HeLa cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg with neutralizing peptide

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody

Predicted band size: 73 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human PPP1R15A aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

O75807

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress (PubMed : 26095357, PubMed : 26742780). Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1 (PubMed : 14718519). May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15' (PubMed : 14635196). Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux (PubMed : 32978159). Acts also a viral restriction factor by attenuating HIV-1 replication (PubMed : 31778897). Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation (PubMed : 31778897).. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR.

See full target information PPP1R15A

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:13575-13592 PubMed41054626

2025

Sirp-α Antibody Inhibits Renal Cell Carcinoma Progression via Akt1/Akt2 Modulation in Tumor-Associated Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Hao,Naiquan Liu,Xin Huang,Hanlei Zhou,Hanrong Li,Yizhou Zhang,Bing Yu,Ziqian Bi,Xinyuan Song,Shunan Li,Keyu Chen,Ning Li,Chao Zhu,Jiahe Wang

Cell death & disease 13:425 PubMed35501353

2022

Mxi1 participates in the progression of lung cancer via the microRNA-300/KLF9/GADD34 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Lei,Yunchao Huang,Jianbin Lin,Shihui Sun,Keda Che,Junting Shen,Jun Liao,Yangming Chen,Kai Chen,Zhaoxian Lin,Xing Lin

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GADD34 抗体

Anti-GADD34 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (AB175355)

Western blot - Anti-GADD34 antibody (AB175355)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise