Anti-GAD65 抗体 [GAD-6] (ab26113)
Key features and details
- Mouse monoclonal [GAD-6] to GAD65
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-GAD65 antibody [GAD-6]
GAD65 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [GAD-6] to GAD65 -
由来種
Mouse -
特異性
The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Mammals -
免疫原
Full length protein (Rat)
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エピトープ
ab26113 recognizes an epitope near the C-terminus of GAD65 -
ポジティブ・コントロール
- mouse brain
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
GAD-6 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab26113の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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特記事項 |
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WB
1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ターゲット情報
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機能
Catalyzes the production of GABA. -
配列類似性
Belongs to the group II decarboxylase family. -
翻訳後修飾
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering. -
細胞内局在
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. - Information by UniProt
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参照データベース
- Entrez Gene: 2572 Human
- Entrez Gene: 14417 Mouse
- Omim: 138275 Human
- SwissProt: Q05329 Human
- SwissProt: P48320 Mouse
- Unigene: 231829 Human
- Unigene: 4784 Mouse
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別名
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE2 antibody
see all
画像
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Anti-GAD65 antibody [GAD-6] (ab26113) at 1/5000 dilution + human brain at 15 µg
Secondary
rabbit anti-mouse-HRP at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa4-12% gradient SDS-PAGE imaged using the pico-plus ECL kit
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Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution + mouse cerebellum at 0.75 µg
Secondary
HRP horse anti-mouse IgG at 1/5000 dilution
Predicted band size: 65 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (76)
ab26113 は 76 報の論文で使用されています。
- Hassan M et al. Developmental Inhibitory Changes in the Primary Somatosensory Cortex of the Stargazer Mouse Model of Absence Epilepsy. Biomolecules 13:N/A (2023). PubMed: 36671571
- Zong FJ et al. Circadian time- and sleep-dependent modulation of cortical parvalbumin-positive inhibitory neurons. EMBO J 42:e111304 (2023). PubMed: 36477886
- Ballon Romero SS et al. Electroacupuncture exerts prolonged analgesic and neuroprotective effects in a persistent dental pain model induced by multiple dental pulp injuries: GABAergic interneurons-astrocytes interaction. Front Immunol 14:1213710 (2023). PubMed: 37954604
- Tomiyama ALMR et al. Neuroprotection by upregulation of the major histocompatibility complex class I (MHC I) in SOD1G93A mice. Front Cell Neurosci 17:1211486 (2023). PubMed: 37711512
- Cheng YC et al. Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte Differentiation. Stem Cell Rev Rep 18:839-852 (2022). PubMed: 35061207