AB26113

Anti-GAD65 抗体 [GAD-6]

Name: Anti-GAD65 抗体 [GAD-6] (ab26113) | Abcam
Brand: Abcam Limited
SKU: AB26113
Price: 72000 JPY
Availability: InStock
Rating: 4 (2 reviews)

Anti-GAD65 antibody [GAD-6]

(2 Reviews)

(89 Publications)

Anti-GAD65 antibody [GAD-6] (ab26113) is a mouse monoclonal antibody detecting GAD65 in Western Blot. Suitable for Human, Mouse.

- Over 70 publications
- Trusted since 2005

別名を表示する

Gad65, Gad2, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, GAD-65

2 Images

Supplier Data

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

All lanes:

Western blot - Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution

All lanes:

mouse cerebellum at 0.75 µg

Secondary

All lanes:

HRP horse anti-mouse IgG at 1/5000 dilution

Predicted band size: 65 kDa

false

CiteAb

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

GAD65 western blot using anti-GAD65 antibody [GAD-6] ab26113. Publication image and figure legend from Woodhouse, A., Fernández-Martos, C. M., et al., 2018, BMC Anesthesiol, PubMed 29699479.

ab26113 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab26113 please see the product overview.

No change in synaptic proteins between propofol and vehicle treated APP/PS1 (n = 3 and 2) and control wild-type (n = 3 for both) mice. Western blot analysis (a) showed no robust change in the expression levels of PSD-95, GAD65/67 and synaptophysin (SYN) in wild-type or APP/PS1 mice treated multiple times with propofol (Prop) or vehicle (VH). Bar graphs show the mean intensity ± SEM of PSD-95 (b), GAD65/67 (c) and synaptophysin (d) normalized to GAPDH

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

GAD-6

アイソタイプ

IgG2a

キャリアフリー

交差種

Mouse

アプリケーション

applications

免疫原

Full Length Protein corresponding to Rat Gad2. The exact immunogen used to generate this antibody is proprietary information.

Q05683

エピトープ

ab26113 recognizes an epitope near the C-terminus of GAD65

特異性

The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-GAD65 antibody [GAD-6] (ab26113) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.

What is the molecular weight of GAD65?
Anti-GAD65 [GAD-6] (ab26113) specifically detects a band for GAD65 (UniProt: Q05329) at a molecular weight of 65kDa.

Trusted by the scientific community
Anti-GAD65 [GAD-6] (ab26113) was first used in a scientific publication in 2005 and has been cited over 70 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

バッファー組成

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the production of GABA.

See full target information Gad2

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文献 (89)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:194 PubMed40713786

2025

Coexistence of CN1A autoantibodies in GAD65 encephalitis exacerbates neurodegeneration : Novel autoantibodies in GAD65 encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Breuer,Delara Kamalizade,Tobias Baumgartner,Juliane L Berns,Thoralf Opitz,Franziska S Thaler,Susanne Schoch,Lars Komorowski,Christoph Helmstaedter,Rainer Surges,Albert J Becker,Julika Pitsch

Pain reports 9:e1209 PubMed39512583

2024

Upregulated spinal histone deacetylases induce nociceptive sensitization by inhibiting the GABA system in chronic constriction injury-induced neuropathy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Hong Wen,Nan-Fu Chen,Hao-Jung Cheng,Hsiao-Mei Kuo,Pei-Yu Chen,Chien-Wei Feng,Zhi-Kang Yao,Wu-Fu Chen,Chun-Sung Sung

The Journal of biological chemistry 300:107779 PubMed39276933

2024

Downregulation of chloride voltage-gated channel 7 contributes to hyperalgesia following spared nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyun Cai,Jiajie Li,Kewei Fan,Dongmei Zhang,Hongjian Lu,Gang Chen

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 21:e00445 PubMed39242290

2024

Extracellular vesicles from mesenchymal stem cells alter gut microbiota and improve neuroinflammation and motor impairment in rats with mild liver damage.

Applications

Unspecified application

Species

Unspecified reactive species

Gergana Mincheva,Vicente Felipo,Victoria Moreno-Manzano,Alfonso Benítez-Páez,Marta Llansola

The journal of venomous animals and toxins including tropical diseases 30:e20230065 PubMed38770186

2024

Effects of local and systemic treatment with human natural killer-1 mimetic peptide (HNK-1) after ventral root avulsion and reimplantation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Scanavachia da Silva,Julia Lombardi,Frank Kirchhoff,Rui Seabra Ferreira,Benedito Barraviera,Alexandre Leite Rodrigues de Oliveira,Luciana Politti Cartarozzi

Frontiers in immunology 14:1213710 PubMed37954604

2023

Electroacupuncture exerts prolonged analgesic and neuroprotective effects in a persistent dental pain model induced by multiple dental pulp injuries: GABAergic interneurons-astrocytes interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Sharmely Sharon Ballon Romero,Lih-Jyh Fuh,Shih-Ya Hung,Yu-Chen Lee,Yu-Chuen Huang,Szu-Yu Chien,Yi-Hung Chen

Frontiers in cellular neuroscience 17:1211486 PubMed37711512

2023

Neuroprotection by upregulation of the major histocompatibility complex class I (MHC I) in SOD1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Laura M R Tomiyama,Luciana Politti Cartarozzi,Lilian de Oliveira Coser,Gabriela Bortolança Chiarotto,Alexandre L R Oliveira

Behavioural brain research 448:114442 PubMed37085118

2023

Evaluations of memory, anxiety, and the growth factor IGF-1R after post-surgical menopause treatment with a highly selective progestin.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria E Bernaud,Stephanie V Koebele,Steven N Northup-Smith,Mari N Willeman,Charlotte Barker,Alex Schatzki-Lumpkin,Maria Valenzuela Sanchez,Heather A Bimonte-Nelson

Biomolecules 13: PubMed36671571

2023

Developmental Inhibitory Changes in the Primary Somatosensory Cortex of the Stargazer Mouse Model of Absence Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Hassan,David R Grattan,Beulah Leitch

Nature communications 13:7805 PubMed36528681

2022

Closed-loop direct control of seizure focus in a rodent model of temporal lobe epilepsy via localized electric fields applied sequentially.

Applications

Unspecified application

Species

Unspecified reactive species

Wonok Kang,Chanyang Ju,Jaesoon Joo,Jiho Lee,Young-Min Shon,Sung-Min Park

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAD65 抗体 [GAD-6]

Anti-GAD65 antibody [GAD-6]

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (89)

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Abcam product promise