Anti-GAD65 抗体 (ab112007)
Key features and details
- Goat polyclonal to GAD65
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GAD65 antibody
GAD65 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GAD65 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog, Pig, Chimpanzee
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免疫原
Synthetic peptide:
C-TLEDNEERMSRLSK
, corresponding to internal sequence amino acids 515-528 of Human GAD65. -
ポジティブ･コントロール
- Human Brain, cerebellum and colon tissues; Human brain lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112007の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 65 kDa.
|
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| IHC-P | (1) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 65 kDa. |
|
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalyzes the production of GABA. -
配列類似性
Belongs to the group II decarboxylase family. -
翻訳後修飾
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering. -
細胞内局在
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. - Information by UniProt
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参照データベース
- Entrez Gene: 466026 Chimpanzee
- Entrez Gene: 487107 Dog
- Entrez Gene: 2572 Human
- Entrez Gene: 14417 Mouse
- Entrez Gene: 396929 Pig
- Entrez Gene: 24380 Rat
- Omim: 138275 Human
- SwissProt: Q4PRC2 Dog
see all -
別名
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody (ab112007)ab112007, at 2.5µg/ml, staining GAD65 in formalin-fixed, paraffin-embedded Human brain, cerebellum tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 antibody (ab112007)ab112007, at 2.5µg/ml, staining GAD65 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. -
Western blot - Anti-GAD65 antibody (ab112007)Anti-GAD65 antibody (ab112007) at 0.1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab112007 は論文での使用が確認できていません。