Anti-GAD65 + GAD67 抗体 (ab49832)
Key features and details
- Rabbit polyclonal to GAD65 + GAD67
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GAD65 + GAD67 antibody
GAD65 + GAD67 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GAD65 + GAD67 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide:
K-DIDFLIEEIERLGQDL
conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 579-594 (with N-terminally added lysine) of Human GAD67. The sequence is identical in Human GAD 65 (amino acids 570-585). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49832の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (2) |
1/1000 - 1/2000.
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特記事項 |
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IHC-P
1/1000 - 1/2000. |
ターゲット情報
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関連性
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome. -
細胞内局在
Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. -
参照データベース
- Entrez Gene: 24379 Rat
- Entrez Gene: 24380 Rat
- SwissProt: P18088 Rat
- SwissProt: Q05683 Rat
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別名
- 65 kDa glutamic acid decarboxylase antibody
- 67 kDa glutamic acid decarboxylase antibody
- GAD 65 antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.
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ab49832 staining human pancreas tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C. An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab49832 は 30 報の論文で使用されています。
- Giehrl-Schwab J et al. Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons. EMBO Mol Med 14:e14797 (2022). PubMed: 35373464
- Struzyna LA et al. Axonal Tract Reconstruction Using a Tissue-Engineered Nigrostriatal Pathway in a Rat Model of Parkinson's Disease. Int J Mol Sci 23:N/A (2022). PubMed: 36430464
- Favrais G et al. Partial protective effects of melatonin on developing brain in a rat model of chorioamnionitis. Sci Rep 11:22167 (2021). PubMed: 34773065
- Grippo RM et al. Dopamine Signaling in the Suprachiasmatic Nucleus Enables Weight Gain Associated with Hedonic Feeding. Curr Biol 30:196-208.e8 (2020). PubMed: 31902720
- Chang W et al. PTH hypersecretion triggered by a GABAB1 and Ca2+-sensing receptor heterocomplex in hyperparathyroidism. Nat Metab 2:243-255 (2020). PubMed: 32694772