Anti-GAD65 + GAD67 抗体 (ab11070)
Key features and details
- Rabbit polyclonal to GAD65 + GAD67
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GAD65 + GAD67 antibody
GAD65 + GAD67 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GAD65 + GAD67 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Pig -
免疫原
Synthetic peptide:
DFLIEEIERLGQDL
, corresponding to C terminal amino acids 572-585 of Human GAD65+67. -
ポジティブ・コントロール
- WB: Mouse brain tissue lysate.
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特記事項
Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. Store undiluted. The product can be stored for up to 12 months. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
An immunoglobulin-rich fraction was prepared from whole serum by ammonium sulphate precipitation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11070の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 65, 67 kDa (predicted molecular weight: 65 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 65, 67 kDa (predicted molecular weight: 65 kDa). |
ターゲット情報
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関連性
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome. -
細胞内局在
Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. -
参照データベース
- Entrez Gene: 14415 Mouse
- Entrez Gene: 14417 Mouse
- Entrez Gene: 396928 Pig
- Entrez Gene: 396929 Pig
- SwissProt: P48318 Mouse
- SwissProt: P48320 Mouse
- SwissProt: 48319 Pig
- SwissProt: P48321 Pig
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別名
- 65 kDa glutamic acid decarboxylase antibody
- 67 kDa glutamic acid decarboxylase antibody
- GAD 65 antibody
see all
画像
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All lanes : Anti-GAD65 + GAD67 antibody (ab11070) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate (negative control)
Secondary
All lanes : Goat Anti-rabbit IgG (HRP)
Predicted band size: 65 kDa
Observed band size: 65,67 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (59)
ab11070 は 59 報の論文で使用されています。
- Huang H et al. FGF3 Directs the Pathfinding of Prethalamic GABAergic Axons. Int J Mol Sci 24:N/A (2023). PubMed: 37834446
- Altbürger C et al. CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain. Front Neuroanat 17:1196868 (2023). PubMed: 37603776
- Kurucu H et al. Inhibitory synapse loss and accumulation of amyloid beta in inhibitory presynaptic terminals in Alzheimer's disease. Eur J Neurol 29:1311-1323 (2022). PubMed: 34331352
- St Pierre M et al. Intrauterine Growth Restriction Disrupts the Postnatal Critical Period of Synaptic Plasticity in the Mouse Dorsal Hippocampus in a Model of Hypertensive Disease of Pregnancy. Dev Neurosci 44:214-232 (2022). PubMed: 34933306
- Tomasello DL et al. 16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis. iScience 25:103551 (2022). PubMed: 34984324