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リコンビナントRabMAb

Anti-GAD1/GAD67 抗体 [EPR20578] - BSA and Azide free (ab246335)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunoprecipitation - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
  • Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20578] to GAD1/GAD67 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free
    GAD1/GAD67 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20578] to GAD1/GAD67 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IP, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse and rat cerebral cortex, cerebellar cortex and pancreas tissues; IP: Human brain lysate; ICC/IF: Mouse primary neuron and rat midbrain neuron cells.

  • 特記事項

    ab246335 is the carrier-free version of ab213508.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab246335の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We don't recommend this antibody for human in IHC. In our hands human tissues showed weak and non-specific staining.
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We don't recommend this antibody for human in IHC. In our hands human tissues showed weak and non-specific staining.

ターゲット情報

  • 機能

    Catalyzes the production of GABA.

  • 組織特異性

    Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

  • 関連疾患

    Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.

  • 配列類似性

    Belongs to the group II decarboxylase family.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 1 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041054 antibody
    • OTTHUMP00000041055 antibody
    • OTTHUMP00000228572 antibody
    • SCP antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunocytochemistry/ Immunofluorescence - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

  • Immunoprecipitation - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunoprecipitation - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    GAD67 was immunoprecipitated from 0.35 mg of human brain lysate with ab213508 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213508 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1:  Human brain lysate 10 µg (Input). 

    Lane 2: ab213508 IP in human brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213508 in human brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

  • Immunocytochemistry/ Immunofluorescence - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunocytochemistry/ Immunofluorescence - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat midbrain neuron cells labelling GAD67 with ab213508 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in rat midbrain neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of mouse pancreas (PMID: 8243826). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebral cortex (PMID: 25904362). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded rat cerebellar cortex tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellar cortex (PMID: 17235515). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GAD67 with ab213508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on islet of rat pancreas (PMID: 8243826). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213508).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)
    Anti-GAD1/GAD67 antibody [EPR20578] - BSA and Azide free (ab246335)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab246335 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab246335 は論文での使用が確認できていません。

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