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AB325669

Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric)

Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric)

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Chicken Monoclonal antibody. Suitable for IHC-P, WB and reacts with Rat samples.

別名を表示する

Gad67, Gad1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with ab325669 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield mounting medium. DAPI (blue) shows cell nuclei. The antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with ab325669 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield mounting medium. DAPI (blue) shows cell nuclei. The antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 100X.

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)
  • WB

Supplier Data

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Western blotting of rat brain homogenate with an anti-GAD65/67 mouse mAb and ab325669. The mouse mAb and ab325669 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The antibody recognizes endogenous GAD1 with similar sensitivity as the anti-GAD65/67 mouse Mab in the rat brain homogenate at 65-67 kDa. GAD1 is an alternative name for GAD65/67.

All lanes:

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (ab325669) at 10 µg/mL

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 65-67 kDa

false

Key facts

宿主種

Chicken

クローン性

Monoclonal

クローン番号

L127/12

アイソタイプ

IgY

キャリアフリー

No

交差種

Rat

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Cross-reacts with GAD25, GAD44 and GAD65/GAD2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L127/12 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L127/12 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
バッファー組成
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.
See full target information Gad1
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