Anti-GABRD 抗体 [EPR25324-253] (ab300348)

製品名

Anti-GABRD antibody [EPR25324-253]
GABRD 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR25324-253] to GABRD
由来種

Rabbit
アプリケーション

適用あり: IHC-Fr, IP, WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF
種交差性

交差種: Mouse, Rat
非交差種: Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Tissue lysates: Mouse cerebral cortex, and cerebellum; Rat cerebellum. IHC-P: Mouse cerebellum and thalamus. Rat brain, cerebellum and thalamus. IHC-Fr.: Mouse and rat cerebellum. IP: Mouse and rat cerebellum.
特記事項
ab300348 does not react in immunocytochemistry and intracellular flow cytometry in human and mous species. Additionally, it does not react in Western Blot and immunohistochemistry in human.

Target Notes:

Gamma-aminobutyric acid receptor subunit delta is specifically expressed in the brain, and highly expressed in the striatum, thalamus and cerebellum, other brain regions are low or not expressed. Subcellular location: cytoplasm and membrane.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR25324-253
アイソタイプ

IgG
研究分野
- Neuroscience
- Processes

Alternative Versions
- Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (ab300351)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300348の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-Fr		1/500.
IP		1/30.
WB		1/1000.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

特記事項
IHC-Fr 1/500.
IP 1/30.
WB 1/1000.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

追加情報

Is unsuitable for Flow Cyt or ICC/IF.

機能

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
関連疾患

Defects in GABRD are the cause of susceptibility to generalized epilepsy with febrile seizures plus type 5 (GEFS+5) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
Defects in GABRD are the cause of susceptibility to idiopathic generalized epilepsy type 10 (IGE10) [MIM:613060]. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
Defects in GABRD are the cause of susceptibility to juvenile myoclonic epilepsy type 7 (EJM7) [MIM:613060]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
配列類似性

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRD sub-subfamily.
細胞内局在

Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Target information above from: UniProt accession O14764 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 14403 Mouse
- Entrez Gene: 29689 Rat
- SwissProt: P22933 Mouse
- SwissProt: P18506 Rat
- Unigene: 388925 Mouse
- Unigene: 10927 Rat
別名
- GABA(A) receptor subunit delta antibody
- Gabrd antibody
- Gamma aminobutyric acid GABA A receptor delta antibody
- Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor antibody
- Gamma-aminobutyric acid receptor subunit delta antibody
- GBRD_HUMAN antibody
- MGC45284 antibody
see all

Western blot - Anti-GABRD antibody [EPR25324-253] (AB300348)

All lanes : Anti-GABRD antibody [EPR25324-253] (ab300348) at 1/1000 dilution

Lane 1 : Mouse cerebral cortex tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat cerebellum tissue lysate
Lane 5 : Rat kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 62 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18408071).

Negative control: kidney (PMID: 12119096)

Application Note: Samples are non-boiled as boiling may cause protein aggregation.

Exposure time: 5.5 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in mouse cerebellum (PMID: 23337532). The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of paraffin-embedded mouse thalamus tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in mouse thalamus (PMID: 23337532). The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat brain. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat cerebellum. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of paraffin-embedded rat thalamus tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat thalamus. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining in mouse kidney. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Frozen sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 (0.956 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: PBS was used instead of primary antibody, followed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2 µg/ml).
Immunohistochemistry (Frozen sections) - Anti-GABRD antibody [EPR25324-253] (AB300348)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 (0.956 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 ug/ml) dilution.
Immunoprecipitation - Anti-GABRD antibody [EPR25324-253] (AB300348)

Gamma-aminobutyric acid receptor subunit delta was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10 µg with ab300348 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300348 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse cerebellum tissue lysate 10 µg (Input)

Lane 2: ab300348 IP in mouse cerebellum tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300348 in mouse cerebellum tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Observed MW (kDa): 62

Exposure time: 3 minutes.
Immunoprecipitation - Anti-GABRD antibody [EPR25324-253] (AB300348)

Gamma-aminobutyric acid receptor subunit delta was immunoprecipitated from 0.35 mg rat cerebellum tissue lysate 10 µg with ab300348 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300348 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Rat cerebellum tissue lysate 10 µg (Input)

Lane 2: ab300348 IP in Rat cerebellum tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300348 in rat cerebellum tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 41 seconds.

Observed MW (kDa): 62.
Anti-GABRD antibody [EPR25324-253] (AB300348)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (0)

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