Anti-GABRA4 抗体 (ab117080)
Key features and details
- Goat polyclonal to GABRA4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GABRA4 antibody
GABRA4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GABRA4 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human GABRA4 aa 300-400 (internal sequence) (Cysteine residue).
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ポジティブ･コントロール
- Human Frontal Cortex lysate and Human Brain, Cortex tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117080の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa.
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| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily. -
細胞内局在
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2557 Human
- Omim: 137141 Human
- SwissProt: P48169 Human
- Unigene: 248112 Human
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別名
- GABA(A) receptor subunit alpha 4 antibody
- GABA(A) receptor subunit alpha-4 antibody
- GABR A4 antibody
see all
画像
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Western blot - Anti-GABRA4 antibody (ab117080)Anti-GABRA4 antibody (ab117080) at 0.1 µg/ml + Human Frontal Cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 62 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRA4 antibody (ab117080)Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab117080 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab117080 は 3 報の論文で使用されています。
- Hughes BA et al. Chronic Ethanol Exposure and Withdrawal Impair Synaptic GABAA Receptor-Mediated Neurotransmission in Deep-Layer Prefrontal Cortex. Alcohol Clin Exp Res 43:822-832 (2019). PubMed: 30860602
- Bohnsack JP et al. Differential regulation of synaptic and extrasynaptic a4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology 105:124-132 (2016). PubMed: 26767953
- Van Skike CE et al. Chronic intermittent ethanol exposure produces persistent anxiety in adolescent and adult rats. Alcohol Clin Exp Res 39:262-71 (2015). PubMed: 25684048