Anti-GABA Transporter 1 / GAT 1 抗体 [EPR12998] (ab177483)
![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.co.jp/ps/products/177/ab177483/Images/ab177483-341537-ab177483.jpg "Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]
GABA Transporter 1 / GAT 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P30531 -
ポジティブ･コントロール
- Fetal brain lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12998 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
ターゲット情報
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機能
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
配列類似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
ドメイン
The PDZ domain-binding motif is involved in the interaction with MPP5. -
細胞内局在
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
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参照データベース
- Entrez Gene: 6529 Human
- Entrez Gene: 232333 Mouse
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P31648 Mouse
- SwissProt: P23978 Rat
- Unigene: 443874 Human
see all -
別名
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
画像
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Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution
Lane 1 : Human brain lysates unboiled
Lane 2 : Human brain lysates boiled
Lane 3 : Mouse brain unboiled
Lane 4 : Mouse brain boiled
Lane 5 : Rat brain unboiled
Lane 6 : Rat brain boiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 to 2: 20 seconds
Lane 3 to 6: 8 secondsWe recommend not to boil the samples after lysis to get desired WB results.
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![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.co.jp/ps/products/177/ab177483/Images/ab177483-205382-anti-gaba-transporter-1-gat-1-antibody-epr12998-western-blot.jpg)
Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg
Predicted band size: 67 kDa -
![Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.co.jp/ps/products/177/ab177483/Images/ab177483-3-benefits-of-recombinant-antibodies.png)
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab177483 は 4 報の論文で使用されています。
- Jin L et al. Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling. Cell Death Dis 13:759 (2022). PubMed: 36055989
- Cai HY et al. Adjusting vascular permeability, leukocyte infiltration, and microglial cell activation to rescue dopaminergic neurons in rodent models of Parkinson's disease. NPJ Parkinsons Dis 7:91 (2021). PubMed: 34625569
- Ren W et al. Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. Mucosal Immunol N/A:N/A (2018). PubMed: 30523310
- Maolakuerban N et al. MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1. Cancer Biol Ther 19:282-291 (2018). PubMed: 29394133