Anti-GABA 抗体 [5A9] (ab86186)
![Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186)](https://www.abcam.co.jp/ps/products/86/ab86186/Images/ab86186-212331-anti-gaba-antibody-5a9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186)")
Key features and details
- Mouse monoclonal [5A9] to GABA
- Suitable for: IHC-P, ICC/IF, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-GABA antibody [5A9]
GABA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5A9] to GABA -
由来種
Mouse -
特異性
ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used. -
アプリケーション
適用あり: IHC-P, ICC/IF, IHC-FoFrmore details -
種交差性
交差種: Species independent -
免疫原
GABA coupled to BSA with glutaraldehyde
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特記事項
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
ポリ/モノ
モノクローナル -
クローン名
5A9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86186の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
|
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| IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell. -
細胞内局在
Cell Membrane, Cytoplasmic, Plasma membrane and Secreted -
別名
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186)Image from Dacks AM et al., PLoS One. 2013;8(7):e69422. Fig 5-F.; doi: 10.1371/journal.pone.0069422.
ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).
Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (ab86186)](https://www.abcam.co.jp/ps/products/86/ab86186/Images/ab86186-280249-anti-gaba-antibody-5a9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (ab86186)Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab86186 は 11 報の論文で使用されています。
- Scheuer T et al. Neonatal Oxidative Stress Impairs Cortical Synapse Formation and GABA Homeostasis in Parvalbumin-Expressing Interneurons. Oxid Med Cell Longev 2022:8469756 (2022). PubMed: 35663195
- Jia X et al. Activation of spinal PDGFRβ in microglia promotes neuronal autophagy via p38 MAPK pathway in morphine-tolerant rats. J Neurochem 158:373-390 (2021). PubMed: 33950542
- Yu XJ et al. Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension. Front Neurosci 15:805070 (2021). PubMed: 34938159
- Trujillo CA et al. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. EMBO Mol Med 13:e12523 (2021). PubMed: 33501759
- Chéreau R et al. Dynamic perceptual feature selectivity in primary somatosensory cortex upon reversal learning. Nat Commun 11:3245 (2020). PubMed: 32591523