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リコンビナントRabMAb

Anti-GABA A Receptor beta 3/GABRB3 抗体 [EPR25323-127] (BSA and Azide free) (ab300064)

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Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
  • Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25323-127] to GABRB3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free)

  • 製品の詳細

    Rabbit monoclonal [EPR25323-127] to GABRB3 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-Fr,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: A549 (human lung carcinoma epithelial cell) whole cell lysate, HEK-293 (human embryonic kidney epithelial cell) whole cell lysate, Mouse cerebellum tissue lysate, Mouse hippocampus tissue lysate, Rat cerebellum tissue lysate, Rat hippocampus tissue lysate, PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate, Rat brain tissue lysate

  • 特記事項

    ab300064 is a carrier free version of ab300063. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25323-127

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300064の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-Fr,IHC-P or IP.

ターゲット情報

  • 機能

    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.

  • 関連疾患

    Epilepsy, childhood absence 5

  • 配列類似性

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily.

  • 細胞内局在

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • GABA(A) receptor subunit beta-3 antibody
      • Gabrb3 antibody
      • Gamma-aminobutyric acid receptor subunit beta-3 antibody
      • GBRB3_HUMAN antibody

    画像

    • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      All lanes : Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (ab300063) at 1/1000 dilution

      Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : U937 (human histiocytic lymphoma monocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 43-54 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      This data was developed using ab300063, the same antibody clone in a different buffer formulation.

      5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

      Samples are non-boiled as boiling may cause protein aggregates.
      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 22303015 ).
      Low expression: HEK-293(PMID:22303015 ), U937 (HPA).

       

    • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      All lanes : Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (ab300063) at 1/1000 dilution

      Lane 1 : Mouse cerebellum tissue lysate
      Lane 2 : Mouse hippocampus tissue lysate
      Lane 3 : Rat cerebellum tissue lysate
      Lane 4 : Rat hippocampus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 54 kDa
      Observed band size: 43-54 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      This data was developed using ab300063, the same antibody clone in a different buffer formulation.

      5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

      Samples are non-boiled as boiling may cause protein aggregates.
      The expression profile/molecular weight observed is consistent with that described in the literature (PMID:22303015).

    • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (ab300063) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa


      Exposure time: 180 seconds


      This data was developed using ab300063, the same antibody clone in a different buffer formulation.

      5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

      Samples are non-boiled as boiling may cause protein aggregates.
      Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (ab300063) at 1/1000 dilution + Rat brain tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 54 kDa
      Observed band size: 43-54 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      This data was developed using ab300063, the same antibody clone in a different buffer formulation.

      5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

      Samples are non-boiled as boiling may cause protein aggregates.
      The expression profile/molecular weight observed is consistent with that described in the literature (PMID:22303015).

    • Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)
      Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300064 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300064 は論文での使用が確認できていません。

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