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Anti-GABA A Receptor alpha 1 抗体 [N95/35] (ab94585)

Submit a review Q&A (1)References (7)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
  • Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)

Key features and details

  • Mouse monoclonal [N95/35] to GABA A Receptor alpha 1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-GABA A Receptor alpha 1 antibody [N95/35]
    GABA A Receptor alpha 1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [N95/35] to GABA A Receptor alpha 1

  • 由来種

    Mouse

  • 特異性

    ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3.

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Cynomolgus monkey, Orangutan

  • 免疫原

    Recombinant fragment within Mouse GABA A Receptor alpha 1 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P62812

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Rat brain normal tissue lysate - membrane extract (ab29473), Human cell line mix lysate. IHC-P: Mouse back skin tissue.

  • 特記事項

    The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab94585の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P
Use a concentration of 0.1 - 1 µg/ml.
ICC/IF
1/50.
特記事項
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P
Use a concentration of 0.1 - 1 µg/ml.
ICC/IF
1/50.

ターゲット情報

  • 機能

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

  • 関連疾患

    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.

  • 配列類似性

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

  • 細胞内局在

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ECA4 antibody
    • EIEE19 antibody
    • EJM antibody
    • EJM5 antibody
    • Gaba receptor alpha 1 polypeptide antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit alpha 1 antibody
    • GABA(A) receptor subunit alpha-1 antibody
    • GABA(A) receptor, alpha 1 antibody
    • GABRA 1 antibody
    • GABRA1 antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
    • Gamma aminobutyric acid A receptor alpha 1 antibody
    • Gamma aminobutyric acid receptor subunit alpha 1 antibody
    • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
    • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
    • GBRA1_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)

    Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.

    Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.

  • Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
    Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
    Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 52 kDa



    Block: 1% BSA.

    Primary antibody incubated for 2 hours at RT.

    Secondary antibody incubated for 1 hour at RT.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (7)

ab94585 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab94585 は 7 報の論文で使用されています。

  • Heiney K  et al. Neuronal avalanche dynamics and functional connectivity elucidate information propagation in vitro. Front Neural Circuits 16:980631 (2022). PubMed: 36188125
  • Zhou Y  et al. Soluble β-amyloid impaired the GABA inhibition by mediating KCC2 in early APP/PS1 mice. Biosci Trends 15:330-340 (2021). PubMed: 34526443
  • Bian Z  et al. Mechanisms Underlying the Action of Ziziphi Spinosae Semen in the Treatment of Insomnia: A Study Involving Network Pharmacology and Experimental Validation. Front Pharmacol 12:752211 (2021). PubMed: 35002696
  • Bu X  et al. 1% Isoflurane and 1.2 µg/ml of Propofol: A Combination of Anesthetics That Causes the Least Damage to Hypoxic Neurons. Front Aging Neurosci 12:591938 (2020). PubMed: 33304268
  • Gong JL  et al. The increased expression of GABA receptors within the arcuate nucleus is associated with high intraocular pressure. Mol Vis 24:574-586 (2018). PubMed: 30174387
  • Zhao J & Hedera P Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners. J Exp Neurosci 9:15-25 (2015). ICC . PubMed: 25987849
  • Tan S  et al. Gene Expression Changes in GABA(A) Receptors and Cognition Following Chronic Ketamine Administration in Mice. PLoS One 6:e21328 (2011). WB ; Mouse . PubMed: 21712993

レビューと Q&A

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レビューを送る 質問を送る

Question

Dear Technical Team,
Hope this e-mail finds you well!
Please find below a complaint from one of our customer to whom we supplied
antibodies having cat nos. ab94585, ab11259, ab124025 & ab31928.
The technical forms are also attached with this mail.
Request you to kindly look into it & advise us accordingly to do further
needful on it.
Looking forward towards your valued reply/ comments on it.
Thanking you in advance.
With Best Regards,

Read More
Answer

Thank you for taking the time to complete our questionnaires and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

To confirm with you, four antibodies are mentioned in your email. However, 2 copies of the questionnaire for ab124025 have been provided and there is no questionnaire for ab11259.

I can provide a response for ab94585,ab124025and ab31928. If you require further help regarding ab11529, please do not hesitate to send the questionnaire and I will be pleased to help.

I would like to reassure you that ab94585 and ab31928 are tested and covered by our 6 month guarantee for use in IHC-Fr and mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement. Regarding ab124025, this is tested and guaranteed in mouse, but has not been tested in frozen sections. Could you confirm if the customer has tried paraffin sections, for which we can provide the guarantee?

Reviewing this case, I would like to offer some suggestions to help optimize the results from ab94585,ab124025and ab31928. I would also appreciate if you can confirm some further details. There are similar comments to provide for all three antibodies:

1. Fixation 1 hour for frozen sections is a very long time. For Frozen sections, we recommend fixation for 10 minutes only. Otherwize there may be too much protein crosslinking from the PFA, and the epitope may not be accessible to the antibody.

2. As far as I am aware, Recoverin, GABA A Receptor alpha 1 and Neurokinin 3 Receptor NK3 are all membrane or membraneassociated protein.Detection of thesewill not require permeabilization. Indeed, Triton is a strong detergent and can disrupt membrane proteins and eptiopes, which can significantly affect the staining. Therefore, I can suggest to remove the permeabilization step.

3. I can recommend to use a more gentle detergent, such as Tween 20 in the other buffers, try 0.1% - 0.2% Tween.

4. I recommend to include 0.1 - 0.2% tween in the wash buffer, this will help to wash away any excess antibody.

5. We recommend not to mix blocking agents in an experiment. try serum only.

6. Could you confirm, are the current vials of secondary antibodies working well with other primary antibodies? What were the results of the no primary controls? The concentration of secondary antibody may need to be reduced to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details, including information regarding ab11259.

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