AB94585

Anti-GABA A Receptor alpha 1 抗体 [N95/35]

Name: Anti-GABA A Receptor alpha 1 抗体 [N95/35] (ab94585) | Abcam
Brand: Abcam Limited
SKU: AB94585
Price: 59000 JPY
Availability: InStock

Anti-GABA A Receptor alpha 1 antibody [N95/35]

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(13 Publications)

Mouse Monoclonal GABA A Receptor alpha 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Gabra1 aa 350-400.

別名を表示する

Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1, GABAAR subunit alpha-1, GABRA1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling GABA A Receptor alpha 1 with ab94585 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha-1 Antibody (D) Composite.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.

Secondary antibody is a FITC Goat Anti-Mouse (green) at 1 : 50 for 1 hour at RT. Localization : Dermal Cells.

Unknown

Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

Block : 1% BSA.

Primary antibody incubated for 2 hours at RT.

Secondary antibody incubated for 1 hour at RT.

All lanes:

Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution

All lanes:

Human cell line mix lysates at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG: HRP

Predicted band size: 52 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

N95/35

アイソタイプ

IgG2a

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Mouse Gabra1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P62812

特異性

ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-10 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.1-1 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by Gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 23909897, PubMed : 25489750, PubMed : 29950725, PubMed : 30602789). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed : 29950725, PubMed : 30602789). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 23909897, PubMed : 29950725, PubMed : 30602789). Alpha-1/GABRA1-containing GABAARs are largely synaptic (By similarity). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (PubMed : 23909897, PubMed : 25489750). GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response (By similarity). GABAARs containing alpha, beta and epsilon subunits also permit spontaneous chloride channel activity while preserving the structural information required for GABA-gated openings (By similarity). Alpha-1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection (By similarity). Together with rho subunits, may also control neuronal and glial GABAergic transmission in the cerebellum (By similarity).

See full target information GABRA1

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of neural transmission (Vienna, Austria : 1996) : PubMed40886229

2025

The effect of recombinant botulinum neurotoxin A on neuropathic pain in the spared nerve injury mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasmus E Hammer,Akinyemi A Omoniyi,Mette Richner,Stephane Lezmi,Christian B Vaegter,Mikhail Kalinichev,Pall Karlsson,Jens R Nyengaard

Research (Washington, D.C.) 8:0746 PubMed40530388

2025

Targeting ApoE-KCC2 Signaling Rescues GABAergic Synaptic Dysfunction and Depression-like Behaviors in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyuan Xu,Jing Liu,Mengru Guo,Jia Wang,Xianbing Bai,Chenlei Zhang,Xinyue Luan,Huailong Pei,Huan Liu,Xinyou Lv,Xiangming Ye,Binliang Tang,Ming Chen

eLife 13: PubMed39565190

2024

NE contribution to rebooting unconsciousness caused by midazolam.

Applications

Unspecified application

Species

Unspecified reactive species

LeYuan Gu,WeiHui Shao,Lu Liu,Qing Xu,YuLing Wang,JiaXuan Gu,Yue Yang,ZhuoYue Zhang,YaXuan Wu,Yue Shen,Qian Yu,XiTing Lian,HaiXiang Ma,YuanLi Zhang,HongHai Zhang

CNS neuroscience & therapeutics 30:e14379 PubMed37545014

2023

Sub-dose anesthetics combined with chloride regulators protect the brain against chronic ischemia-hypoxia injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyi Yang,Ye Wang,Yun Li,Xinyi Wang,Wei Hua,Zhuo Yang,Haiyun Wang

Frontiers in neural circuits 16:980631 PubMed36188125

2022

Neuronal avalanche dynamics and functional connectivity elucidate information propagation .

Applications

Unspecified application

Species

Unspecified reactive species

Kristine Heiney,Ola Huse Ramstad,Vegard Fiskum,Axel Sandvig,Ioanna Sandvig,Stefano Nichele

Frontiers in pharmacology 12:752211 PubMed35002696

2021

Mechanisms Underlying the Action of Ziziphi Spinosae Semen in the Treatment of Insomnia: A Study Involving Network Pharmacology and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Bian,Wenming Zhang,Jingyue Tang,Qianqian Fei,Minmin Hu,Xiaowei Chen,Lianlin Su,Chenghao Fei,De Ji,Chunqin Mao,Huangjin Tong,Xiaohang Yuan,Tulin Lu

Bioengineered 12:11823-11836 PubMed34719333

2021

Effect and mechanism of BDNF/TrkB signaling on vestibular compensation.

Applications

Unspecified application

Species

Unspecified reactive species

Dehong Mao,Zhongmei He,Wei Xuan,Jiao Deng,Weichun Li,Xiaoying Fang,Linglong Li,Feng Zhang

Bioscience trends 15:330-340 PubMed34526443

2021

Soluble β-amyloid impaired the GABA inhibition by mediating KCC2 in early APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhou,Yujie Cheng,Yong Li,Jiyao Ma,Zhihan Wu,Yuenan Chen,Jinyu Mei,Ming Chen

Frontiers in aging neuroscience 12:591938 PubMed33304268

2020

1% Isoflurane and 1.2 μg/ml of Propofol: A Combination of Anesthetics That Causes the Least Damage to Hypoxic Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Bu,Tang Li,Di Guo,Chenyi Yang,Jinxin Wang,Xinyi Wang,Zhuo Yang,Haiyun Wang

Molecular vision 24:574-586 PubMed30174387

2018

The increased expression of GABA receptors within the arcuate nucleus is associated with high intraocular pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Ling Gong,Xiao-Tong Lou,Yu-Xiang Yuan,Li-Wen Chen,Ping-Ting Ji,Lu Li,Yin Zhao,Hong Zhang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA A Receptor alpha 1 抗体 [N95/35]

Anti-GABA A Receptor alpha 1 antibody [N95/35]

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

Western blot - Anti-GABA A Receptor alpha 1 antibody [N95/35] (AB94585)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (13)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise