Anti-GAB2 (phospho Y643) 抗体 (ab78262)
Key features and details
- Rabbit polyclonal to GAB2 (phospho Y643)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GAB2 (phospho Y643) antibody
GAB2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GAB2 (phospho Y643) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human GAB2 (phospho Y643).
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ポジティブ･コントロール
- Extracts from Jurkat cells. Human brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78262の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis. -
配列類似性
Belongs to the GAB family.
Contains 1 PH domain. -
ドメイン
The SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding. -
翻訳後修飾
Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11. -
細胞内局在
Cytoplasm. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9846 Human
- Entrez Gene: 14389 Mouse
- Entrez Gene: 84477 Rat
- Omim: 606203 Human
- SwissProt: Q9UQC2 Human
- SwissProt: Q9Z1S8 Mouse
- SwissProt: Q9EQH1 Rat
- Unigene: 429434 Human
see all -
別名
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
see all
画像
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Western blot - Anti-GAB2 (phospho Y643) antibody (ab78262)All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins)
Lane 2 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB2 (phospho Y643) antibody (ab78262)Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab78262 は 1 報の論文で使用されています。
- Yim JH et al. Phosphoprotein profiles of candidate markers for early cellular responses to low-dose ?-radiation in normal human fibroblast cells. J Radiat Res 58:329-340 (2017). WB ; Human . PubMed: 28122968