Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-GAB2 抗体 [EP369Y] (ab32365)

Submit a review Q&A (2)References (4)
Western blot - Anti-GAB2 antibody [EP369Y] (ab32365)
  • Anti-GAB2 antibody [EP369Y] (ab32365)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP369Y] to GAB2
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-GAB2 antibody [EP369Y]
    GAB2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP369Y] to GAB2

  • 由来種

    Rabbit

  • 特異性

    This antibody is predicted to detect the splice isoform 2 based on sequence homology.

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF,IHC or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human GAB2 aa 600-700. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: K562 cell lysates

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32365の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
特記事項
WB
1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
追加情報
Is unsuitable for ICC/IF,IHC or IP.

ターゲット情報

  • 機能

    Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

  • 配列類似性

    Belongs to the GAB family.
    Contains 1 PH domain.

  • ドメイン

    The SH3-binding motifs mediate interaction with SHC1 and GRB2.
    The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.

  • 翻訳後修飾

    Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
    Dephosphorylated by PTPN11.

  • 細胞内局在

    Cytoplasm. Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GAB 2 antibody
    • Gab2 antibody
    • GAB2_HUMAN antibody
    • Grb 2 associated binder 2 antibody
    • GRB 2 associated binding protein 2 antibody
    • Grb2 associated binder 2 antibody
    • GRB2 associated binder 2 pp100 antibody
    • GRB2 associated binding protein 2 antibody
    • GRB2-associated binder 2 antibody
    • GRB2-associated-binding protein 2 antibody
    • Growth factor receptor bound protein 2 associated protein 2 antibody
    • Growth factor receptor bound protein 2-associated protein 2 antibody
    • KIAA0571 antibody
    • p97 antibody
    • PH domain containing adaptor molecule p97 antibody
    • pp100 antibody
    see all

画像

  • Western blot - Anti-GAB2 antibody [EP369Y] (ab32365)
    Western blot - Anti-GAB2 antibody [EP369Y] (ab32365)
    All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in RIPA lysis method
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in 1% SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    Blocking/Diluting Buffer and concentration:
    5% NFDM/TBST

  • Anti-GAB2 antibody [EP369Y] (ab32365)
    Anti-GAB2 antibody [EP369Y] (ab32365)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab32365 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32365 は 4 報の論文で使用されています。

  • Sun J  et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
  • Mu L  et al. MicroRNA-218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2. Oncol Rep 44:1961-1970 (2020). PubMed: 32901879
  • Tian LQ  et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . PubMed: 27035789
  • Pyarajan S  et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . PubMed: 18586679

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Dear Sirs,

I would like to query your technical support regarding the following antibodies:
Ab62802,ab32365, ab78262 and ab92578.
In order to detect changes in GAB2 and phospho-GAB2 levels we ordered several anti GAB2 and anti phospho-GAB2 antibodies from different sources.
After trying several cell lysis conditions to minimize breakdown products, we settled for the cell lysis buffer by Cell signaling technologies (cat #3884 in combination with a protease inhibitor cocktail from Roche). Result where generated using the K562 cell line which is also mentioned in the datasheet of Ab62802,ab32365 and ab92578
Using the competitors antibodies we are able to assure the availability of both GAB2 and phosphorylation site specific forms of phospho-GAB2.
Unfortunately using the anti GAB2 antibodies ab62802,ab32365,ab92578 and ab78262 we are not able to get the desired results.
Ab62802 does not yield a nice and clean band at the expected 80 to 90 KDa, instead we see a smear/aspecific binding to our membrane.
The antibodies ab32365,ab92578 and ab78262 do not yield a band when using either lysates of K562 or IFN triggered Jurkat cells (according to the ab78262 datasheet).
Since the competitors antibodies clearly show the availability of both GAB2 and pGAB2 in the K562 cell line we seriously doubt the effectiveness of the Ab62802,ab32365, ab78262 and ab92578 antibodies (please see attached pdf for a line up of the antibodies used (markings in blue are competitor products).
Our question is, do you have any suggestion to alleviate our problems, we are somewhat at a loss here.
Kind regards,

Read More
Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to better understand the problem, I’d like to send you a questionnaire with some questions about the protocol used.

Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results.

According to the image sent the three anti phosphorilated GAB2 antibodies show different patterns, both #1 and #3 do not show any bands and #2 however gives several bands. Are the three antibodies targeting the same phosphorilated site in the protein?

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question


Inquiry: Do you cell a blocking peptide for GAB2 antibody?

Read More
Answer

Hello! The blocking peptides for these antibodies have now been published in our catalog, they are ab133951 and ab133952. Please let me know if you have any further questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com