Anti-G-protein coupled receptor 30 抗体 - C-terminal (ab154069)
Key features and details
- Rabbit polyclonal to G-protein coupled receptor 30 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-G-protein coupled receptor 30 antibody - C-terminal
G-protein coupled receptor 30 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to G-protein coupled receptor 30 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 307-375 of Human G-protein coupled receptor 30 (Uniprot: Q99527).
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ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and RL952 whole cell lysates; Human endometrial tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154069の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor for estrogen. -
組織特異性
Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor. - Information by UniProt
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参照データベース
- Entrez Gene: 2852 Human
- Omim: 601805 Human
- SwissProt: Q99527 Human
- Unigene: 20961 Human
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別名
- CEPR antibody
- Chemoattractant receptor-like 2 antibody
- Chemokine receptor-like 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069)Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.
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Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069)All lanes : Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS PAGE -
Western blot - Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069)All lanes : Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069) at 1/10000 dilution
Lane 1 : RL952 whole cell lysate
Lane 2 : RL952 whole cell lysate; transfected with G-protein coupled receptor 30 shRNA
Lysates/proteins at 40 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody - C-terminal (ab154069)Immunohistochemical analysis of paraffin-embedded Human endometrial tissue labeling G-protein coupled receptor 30 with ab154069 at 1/150 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab154069 は 14 報の論文で使用されています。
- Bühler M et al. GPER1 links estrogens to centrosome amplification and chromosomal instability in human colon cells. Life Sci Alliance 6:N/A (2023). PubMed: 36384894
- Gallo Cantafio ME et al. GPER1 Activation Exerts Anti-Tumor Activity in Multiple Myeloma. Cells 12:N/A (2023). PubMed: 37759449
- Song T et al. Resveratrol stimulates StAR expression and progesterone production by GPER-mediated downregulation of Snail expression in human granulosa cells. J Food Drug Anal 31:315-325 (2023). PubMed: 37335164
- Abancens M et al. GPER Agonist G1 Prevents Wnt-Induced JUN Upregulation in HT29 Colorectal Cancer Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36293473
- Zhang R et al. GPR30 knockdown weakens the capacity of CAF in promoting prostate cancer cell invasion via reducing macrophage infiltration and M2 polarization. J Cell Biochem N/A:N/A (2021). PubMed: 33938030