Anti-G protein beta subunit like/GBL 抗体 (ab25974)
Key features and details
- Rabbit polyclonal to G protein beta subunit like/GBL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-G protein beta subunit like/GBL antibody
G protein beta subunit like/GBL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to G protein beta subunit like/GBL -
由来種
Rabbit -
特異性
ab25974 recognises GßL (G protein beta protein subunit-like). -
アプリケーション
適用あり: WBmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human G protein beta subunit like/GBL (C terminal).
(Peptide available asab39766) -
ポジティブ・コントロール
- WB: Human brain cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25974の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like/GBL peptide (ab39766).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Can be blocked with Human G protein beta subunit like/GBL peptide (ab39766). |
ターゲット情報
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機能
Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. -
組織特異性
Broadly expressed, with highest levels in skeletal muscle, heart and kidney. -
配列類似性
Belongs to the WD repeat LST8 family.
Contains 7 WD repeats. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 64223 Human
- Omim: 612190 Human
- SwissProt: Q9BVC4 Human
- Unigene: 29203 Human
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別名
- G protein beta subunit like antibody
- G protein beta subunit-like antibody
- Gable antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab25974 は 1 報の論文で使用されています。
- Lin Z et al. Construction of competitive endogenous RNA network reveals regulatory role of long non-coding RNAs in type 2 diabetes mellitus. J Cell Mol Med 21:3204-3213 (2017). PubMed: 28643459