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リコンビナントRabMAb

Anti-G-CSF 抗体 [EPR3203(N)(B)] (ab181053)

Reviews (1) Submit a question References (10)
Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
  • Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3203(N)(B)] to G-CSF
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

製品の概要

  • 製品名

    Anti-G-CSF antibody [EPR3203(N)(B)]
    G-CSF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3203(N)(B)] to G-CSF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: A549, K-562, HepG2, HeLa, MCF7, MOLT-4, PC-3, KM3, NCI-H460 and HT-1376 cell lysates; Mouse and rat brain lysates. ICC/IF: BxPC-3 and HT-1376 cells. IP: G-CSF IP in K562 cell lysate. Flow Cyt (intra): K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181053の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/200.
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100 dilution.
IP
1/100.

For unpurified use at 1/20 dilution. 
特記事項
Flow Cyt (Intra)
1/200.
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100 dilution.
IP
1/100.

For unpurified use at 1/20 dilution. 

ターゲット情報

  • 機能

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

  • 配列類似性

    Belongs to the IL-6 superfamily.

  • 翻訳後修飾

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C17orf33 antibody
      • Colony stimulating factor 3 (granulocyte) antibody
      • Colony stimulating factor 3 antibody
      • CSF 3 antibody
      • CSF beta antibody
      • CSF3 antibody
      • CSF3_HUMAN antibody
      • CSF3OS antibody
      • Csfg antibody
      • Filgrastim antibody
      • G-CSF antibody
      • GCSA antibody
      • GCSF antibody
      • Granulocyte colony stimulating factor antibody
      • Granulocyte colony-stimulating factor antibody
      • Lenograstim antibody
      • Macrophage granulocyte inducer 2 antibody
      • MGC45931 antibody
      • MGI 2 antibody
      • Pluripoietin antibody
      see all

    画像

    • Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      All lanes : Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/1000 dilution

      Lane 1 : A549 (Human lung carcinoma epithelial cell) cell lysate
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate
      Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate
      Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
      Lane 5 : MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate
      Lane 6 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate
      Lane 7 : PC-3 (Human prostate adenocarcinoma epithelial cell) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 22 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

        

       Exposure time: 4 s

    • Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Flow Cytometry (Intracellular) - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)

      Intracellular Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling G-CSF with purified ab181053 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)

      Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cells labeling G-CSF with Purified ab181053 at 1:500 dilution (4.0μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear was used as a counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)

      ab181053 (purified) at 1:100 dilution (2ug) immunoprecipitating G-CSF in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
      Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10ug
      Lane 2 (+): ab181053 & K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181053 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST."

    • Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      All lanes : Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/2000 dilution (unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : KM3 cell lysate
      Lane 3 : NCI-H460 cell lysate
      Lane 4 : HT-1376 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

      Predicted band size: 22 kDa

    • Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      All lanes : Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/2000 dilution (purified)

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 2 : HT-1376 (Human urinary bladder carcinoma epithelial cell) whole cell lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 22 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)

      Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling G-CSF with unpurified ab181053 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    • Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)

      Western blot analysis of immunoprecipitation pellet from K562 cell lysate (lane 1) or a Negative control (lane 2)  immunoprecipitated using unpurified ab181053 at 1/20 dilution.

      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)
      Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053)

    参考文献 (10)

    ab181053 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181053 は 10 報の論文で使用されています。

    • Wen H  et al. Ruscogenins Improve CD-Like Enteritis by Inhibiting Apoptosis of Intestinal Epithelial Cells and Activating Nrf2/NQO1 Pathway. Oxid Med Cell Longev 2022:4877275 (2022). PubMed: 35308175
    • Zhang W  et al. Suppression of Aspergillus fumigatus Germination by Neutrophils Is Enhanced by Endothelial-Derived CSF3 Production. Front Microbiol 13:837776 (2022). PubMed: 35572651
    • Wang X  et al. Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells. Cancer Gene Ther 29:1742-1750 (2022). PubMed: 35768562
    • Yu H  et al. Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis. BMC Oral Health 21:299 (2021). PubMed: 34118920
    • Touzelet O  et al. The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics 19:793-807 (2020). PubMed: 32075873
    • Fang C  et al. CSF3 Is a Potential Drug Target for the Treatment of COVID-19. Front Physiol 11:605792 (2020). PubMed: 33551833
    • Calipari ES  et al. Granulocyte-colony stimulating factor controls neural and behavioral plasticity in response to cocaine. Nat Commun 9:9 (2018). PubMed: 29339724
    • Wang X  et al. Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway. Cell Death Dis 9:578 (2018). PubMed: 29784961
    • Pasquier J  et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). PubMed: 29180664
    • Lu W  et al. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling. Sci Rep 7:8402 (2017). PubMed: 28827748

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody [EPR3203(N)(B)]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mesothelioma tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Permeabilization
    Yes - 0.4% triton
    Specification
    mesothelioma tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. inbal mis

    Verified customer

    投稿 Jan 21 2019

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