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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Fyn 抗体 [EPR19636] (ab184276)

Submit a review Submit a question References (10)
Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
  • Flow Cytometry (Intracellular) - Anti-Fyn antibody [EPR19636] (ab184276)
  • Immunoprecipitation - Anti-Fyn antibody [EPR19636] (ab184276)
  • Anti-Fyn antibody [EPR19636] (ab184276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19636] to Fyn
  • Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-Fyn antibody [EPR19636]
    Fyn 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19636] to Fyn

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human brain lysate; Ramos, HEK-293, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; P0 mouse brain lysate; P0 rat brain lysate; Rat spleen lysate. IHC-P: Human tonsil and cerebral cortex tissues; Mouse spleen tissue; Rat testis tissue. Flow Cyt (intra): RAW 264.7 cells. IP: P0 mouse brain lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184276の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.
Flow Cyt (Intra)
1/50.
特記事項
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40.
Flow Cyt (Intra)
1/50.

ターゲット情報

  • 機能

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.

  • 組織特異性

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • 細胞内局在

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

  • 別名

    • C syn protooncogene antibody
    • Fyn antibody
    • FYN oncogene related to SRC FGR YES antibody
    • FYN_HUMAN antibody
    • OKT3 induced calcium influx regulator antibody
    • P59 FYN antibody
    • p59-Fyn antibody
    • Protein tyrosine kinase fyn antibody
    • Proto oncogene tyrosine protein kinase fyn antibody
    • Proto-oncogene c-Fyn antibody
    • Proto-oncogene Syn antibody
    • Protooncogene Syn antibody
    • SLK antibody
    • Src like kinase antibody
    • Src yes related novel gene antibody
    • Src-like kinase antibody
    • Src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn T antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine-protein kinase Fyn antibody
    see all

画像

  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    All lanes : Anti-Fyn antibody [EPR19636] (ab184276) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 cell lysate
    Lane 2 : FYN knockout HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-Fyn antibody [EPR19636] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab184276 was shown to bind specifically to Fyn. A band was observed at 60 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in FYN knockout cell line ab269630 (knockout cell lysate ab272440). To generate this image, wild-type and FYN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    All lanes : Anti-Fyn antibody [EPR19636] (ab184276) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : FYN knockout HEK-293T cell lysate
    Lane 3 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 159 kDa why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab184276 observed at 60 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab184276 Anti-Fyn antibody [EPR19636] was shown to specifically react with Fyn in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266133 (knockout cell lysate ab257071) was used. Wild-type and Fyn knockout samples were subjected to SDS-PAGE. ab184276 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    All lanes : Anti-Fyn antibody [EPR19636] (ab184276) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : P0 mouse brain lysate
    Lane 5 : P0 rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 2: 3 minutes; Lanes 3,4 and 5: 8 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on T cells of human tonsil (PMID: 10523617). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human cerebral cortex (PMID: 7544314). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on T cells of mouse spleen (PMID: 7544314, PMID:10523617). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [EPR19636] (ab184276)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Fyn with ab184276 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on rat testis (PMID: 7544314). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    Western blot - Anti-Fyn antibody [EPR19636] (ab184276)
    All lanes : Anti-Fyn antibody [EPR19636] (ab184276) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-Fyn antibody [EPR19636] (ab184276)
    Flow Cytometry (Intracellular) - Anti-Fyn antibody [EPR19636] (ab184276)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling Fyn with ab184276 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-Fyn antibody [EPR19636] (ab184276)
    Immunoprecipitation - Anti-Fyn antibody [EPR19636] (ab184276)

    Fyn was immunoprecipitated from 0.35 mg of P0 mouse brain lysate with ab184276 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184276 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: P0 mouse brain lysate 10 µg (Input). 

    Lane 2: ab184276 IP in P0 mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184276 in P0 mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Fyn antibody [EPR19636] (ab184276)
    Anti-Fyn antibody [EPR19636] (ab184276)

参考文献 (10)

ab184276 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184276 は 10 報の論文で使用されています。

  • Wang T  et al. Proteomics analysis in myocardium of spontaneously hypertensive rats. Sci Rep 13:276 (2023). PubMed: 36609626
  • Chen L  et al. ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation. Cell Biosci 13:42 (2023). PubMed: 36855057
  • Peng S  et al. FYN/TOPK/HSPB1 axis facilitates the proliferation and metastasis of gastric cancer. J Exp Clin Cancer Res 42:80 (2023). PubMed: 37016377
  • Chen M  et al. CD36 regulates diurnal glucose metabolism and hepatic clock to maintain glucose homeostasis in mice. iScience 26:106524 (2023). PubMed: 37123238
  • Yang Y  et al. FYN regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specialization via its effect on Arp3 in the mouse testis. Front Immunol 13:915274 (2022). PubMed: 36016954
  • Duan H  et al. CD146 bound to LCK promotes T cell receptor signaling and antitumor immune responses in mice. J Clin Invest 131:N/A (2021). PubMed: 34491908
  • Tan C  et al. Fyn kinase regulates dopaminergic neuronal apoptosis in animal and cell models of high glucose (HG) treatment. BMC Mol Cell Biol 22:58 (2021). PubMed: 34863087
  • Luo X  et al. Fyn gene silencing reduces oligodendrocytes apoptosis through inhibiting ERK1/2 phosphorylation in epilepsy. Artif Cells Nanomed Biotechnol 48:298-304 (2020). PubMed: 31852295
  • Tang Y  et al. Bioinformatic analysis of differentially expressed genes and identification of key genes in EBV-transformed lymphoblasts. Biomed Pharmacother 116:108984 (2019). PubMed: 31129512
  • Zambrano S  et al. GPRC5b Modulates Inflammatory Response in Glomerular Diseases via NF-?B Pathway. J Am Soc Nephrol 30:1573-1586 (2019). PubMed: 31285284

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