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リコンビナントRabMAb

Anti-FYB 抗体 [EP2546Y] (ab76103)

Reviews (2) Submit a question References (1)
Western blot - Anti-FYB antibody [EP2546Y] (ab76103)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EP2546Y] (ab76103)
  • Flow Cytometry (Intracellular) - Anti-FYB antibody [EP2546Y] (ab76103)
  • Anti-FYB antibody [EP2546Y] (ab76103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2546Y] to FYB
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-FYB antibody [EP2546Y]
    FYB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2546Y] to FYB

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human FYB aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: O15117

  • ポジティブ･コントロール

    • WB: TF-1 lysate. IHC-P: Human tonsil tissue. Flow Cyt (intra): TF-1 cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP2546Y

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76103の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/5000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/5000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.

  • 組織特異性

    Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.

  • 配列類似性

    Contains 1 SH3 domain.

  • 翻訳後修飾

    T-cell receptor ligation leads to increased tyrosine phosphorylation.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ADAP antibody
      • Adhesion and degranulation promoting adaptor protein antibody
      • FYB 120/130 antibody
      • Fyb antibody
      • FYB-120/130 antibody
      • FYB_HUMAN antibody
      • FYN binding protein antibody
      • FYN T binding protein antibody
      • FYN-binding protein antibody
      • FYN-T-binding protein antibody
      • p120/p130 antibody
      • PRO0823 antibody
      • SLAP 130 antibody
      • SLAP-130 antibody
      • SLAP130 antibody
      • SLP 76 associated phosphoprotein antibody
      • SLP-76-associated phosphoprotein antibody
      • SLP76 associated phosphoprotein antibody
      see all

    画像

    • Western blot - Anti-FYB antibody [EP2546Y] (ab76103)
      Western blot - Anti-FYB antibody [EP2546Y] (ab76103)
      Anti-FYB antibody [EP2546Y] (ab76103) at 1/10000 dilution + TF-1 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 85 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EP2546Y] (ab76103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EP2546Y] (ab76103)

      ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-FYB antibody [EP2546Y] (ab76103)
      Flow Cytometry (Intracellular) - Anti-FYB antibody [EP2546Y] (ab76103)

      Intracellular Flow Cytometry analysis of TF-1 (Human Erythroleukemia erythroblast) cells labeling FYB with purified ab76103 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

       

    • Anti-FYB antibody [EP2546Y] (ab76103)
      Anti-FYB antibody [EP2546Y] (ab76103)

    参考文献 (1)

    ab76103 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76103 は 1 報の論文で使用されています。

    • Zhang K  et al. Super Enhancer Regulatory Gene FYB1 Promotes the Progression of T Cell Acute Lymphoblastic Leukemia by Activating IGLL1. J Immunol Res 2023:3804605 (2023). PubMed: 37767202

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FYB antibody [EP2546Y]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (CHO cell expressing human ADAP)
    Specification
    CHO cell expressing human ADAP
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.2% Triton X100, 5 min
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 04 2011

    Western blot abreview for Anti-FYB antibody [EP2546Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (transfected CHO cells)
    Loading amount
    40 µg
    Specification
    transfected CHO cells
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 08 2010

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