Anti-FXYD1/PLM 抗体 (ab76597)
Key features and details
- Rabbit polyclonal to FXYD1/PLM
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FXYD1/PLM antibody
FXYD1/PLM 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FXYD1/PLM -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Macaque monkey
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免疫原
Synthetic peptide corresponding to Human FXYD1/PLM aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab88161) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
ターゲット情報
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機能
May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. -
組織特異性
Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis. -
配列類似性
Belongs to the FXYD family. -
翻訳後修飾
Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.
Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 476487 Dog
- Entrez Gene: 5348 Human
- Entrez Gene: 56188 Mouse
- Entrez Gene: 58971 Rat
- Omim: 602359 Human
- SwissProt: P56513 Dog
- SwissProt: O00168 Human
- SwissProt: Q9Z239 Mouse
see all -
別名
- FXYD domain containing ion transport regulator 1 antibody
- FXYD domain-containing ion transport regulator 1 antibody
- FXYD1 antibody
see all
画像
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Western blot - Anti-FXYD1/PLM antibody (ab76597)All lanes : Anti-FXYD1/PLM antibody (ab76597) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 10 kDa
Observed band size: 10 kDa
Exposure time: 5 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab76597 は 11 報の論文で使用されています。
- Main A et al. Dynamic but discordant alterations in zDHHC5 expression and palmitoylation of its substrates in cardiac pathologies. Front Physiol 13:1023237 (2022). PubMed: 36277202
- Bubb KJ et al. FXYD1 Is Protective Against Vascular Dysfunction. Hypertension 77:2104-2116 (2021). PubMed: 33934624
- Meyer DJ et al. FXYD protein isoforms differentially modulate human Na/K pump function. J Gen Physiol 152:N/A (2020). PubMed: 33231612
- Wang X et al. Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level. Cell Res 30:1109-1126 (2020). PubMed: 32690901
- Plain F et al. Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase. Commun Biol 3:411 (2020). PubMed: 32737405