Anti-FXR1 抗体 (ab51970)
Key features and details
- Goat polyclonal to FXR1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FXR1 antibody
FXR1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to FXR1 -
由来種
Goat -
特異性
This antibody is expected to recognise all reported isoforms -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Dog
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免疫原
Synthetic peptide:
RIEGDNENKLPRED
, corresponding to amino acids 317/330 of Human FXR1 -
ポジティブ･コントロール
- WB: NIH/3T3 cell lysate. ICC/IF: HeLa and A431 cells. IHC-P: Human skin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51970の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
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| WB | (2) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. -
組織特異性
Expressed in all tissues examined including heart, brain, kidney and testis. -
配列類似性
Belongs to the FMR1 family.
Contains 2 KH domains. -
翻訳後修飾
Arg-445 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8087 Human
- Entrez Gene: 14359 Mouse
- Entrez Gene: 361927 Rat
- Omim: 600819 Human
- SwissProt: P51114 Human
- SwissProt: Q61584 Mouse
- SwissProt: Q5XI81 Rat
- Unigene: 478407 Human
see all -
別名
- Fragile X mental retardation autosomal homolog 1 antibody
- Fragile X mental retardation syndrome related protein 1 antibody
- Fragile X mental retardation syndrome-related protein 1 antibody
see all
画像
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Western blot - Anti-FXR1 antibody (ab51970)Anti-FXR1 antibody (ab51970) at 0.2 µg/ml + NIH/3T3 cell lysate in RIPA buffer at 35 µg
Predicted band size: 69 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody (ab51970)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody (ab51970)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody (ab51970)ICC/IF image of ab51970 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab51970 は 6 報の論文で使用されています。
- Roy N et al. mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activation. Skelet Muscle 11:18 (2021). PubMed: 34238354
- Zhao L et al. A Clostridia-rich microbiota enhances bile acid excretion in diarrhea-predominant irritable bowel syndrome. J Clin Invest 130:438-450 (2020). PubMed: 31815740
- Nordio L et al. Immunohistochemical Expression of FXR1 in Canine Normal Tissues and Melanomas. J Histochem Cytochem N/A:22155418766292 (2018). PubMed: 29608406
- Oldenburg AR et al. Deregulation of Fragile X-related protein 1 by the lipodystrophic lamin A p.R482W mutation elicits a myogenic gene expression program in preadipocytes. Hum Mol Genet N/A:N/A (2013). ICC/IF ; Human . PubMed: 24108105
- Xu XL et al. FXR1P But Not FMRP Regulates the Levels of Mammalian Brain-Specific microRNA-9 and microRNA-124. J Neurosci 31:13705-9 (2011). PubMed: 21957233
- Khera TK et al. Fragile X-related protein FXR1 controls post-transcriptional suppression of lipopolysaccharide-induced tumour necrosis factor-alpha production by transforming growth factor-beta1. FEBS J 277:2754-65 (2010). WB ; Mouse . PubMed: 20491901