Anti-FXR1 抗体 (ab245624)
Key features and details
- Rabbit polyclonal to FXR1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FXR1 antibody
FXR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FXR1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human FXR1 aa 571-621. The exact sequence is proprietary. NP_005078.2
Database link: P51114 -
ポジティブ･コントロール
- WB: HEK-293T, HeLa, 3T3 and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245624 was affinity purified using an epitope specific to FXR1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245624の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 70 kDa. |
ターゲット情報
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機能
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. -
組織特異性
Expressed in all tissues examined including heart, brain, kidney and testis. -
配列類似性
Belongs to the FMR1 family.
Contains 2 KH domains. -
翻訳後修飾
Arg-445 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8087 Human
- Entrez Gene: 14359 Mouse
- Omim: 600819 Human
- SwissProt: P51114 Human
- SwissProt: Q61584 Mouse
- Unigene: 478407 Human
- Unigene: 259021 Mouse
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別名
- Fragile X mental retardation autosomal homolog 1 antibody
- Fragile X mental retardation syndrome related protein 1 antibody
- Fragile X mental retardation syndrome-related protein 1 antibody
see all
画像
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Immunoprecipitation - Anti-FXR1 antibody (ab245624)
FXR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245624 at 6 μg/reaction. Western blot was perfromed on the immunoprecipitate using ab245624 at 1 μg/ml.
Lane 1: ab245624 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Western blot - Anti-FXR1 antibody (ab245624)All lanes : Anti-FXR1 antibody (ab245624) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes -
Western blot - Anti-FXR1 antibody (ab245624)All lanes : Anti-FXR1 antibody (ab245624) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245624 は論文での使用が確認できていません。