Anti-FUZ 抗体 (ab111842)
Key features and details
- Rabbit polyclonal to FUZ
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FUZ antibody
FUZ 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FUZ -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 76-348 of Human FUZ (AAH04445).
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ポジティブ･コントロール
- HCT116 whole cell lysate; Human Gastric carcinoma and RT2 xenograft tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab111842 is purified by antigen-affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111842の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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| IHC-P |
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
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IHC-P
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
ターゲット情報
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機能
Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. May regulate the morphogenesis of hair follicles which depends on functional primary cilia. -
配列類似性
Belongs to the fuzzy family. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 80199 Human
- Entrez Gene: 70300 Mouse
- Entrez Gene: 308577 Rat
- Omim: 610622 Human
- SwissProt: Q9BT04 Human
- SwissProt: Q3UYI6 Mouse
- SwissProt: Q3B756 Rat
- Unigene: 288800 Human
see all -
別名
- FLJ22688 antibody
- fuz antibody
- FUZZY_HUMAN antibody
see all
画像
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Western blot - Anti-FUZ antibody (ab111842)Anti-FUZ antibody (ab111842) at 1/1000 dilution + HCT116 cell line lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (ab111842)ab111842, at 1/500 dilution, staining FUZ in Formalin-fixed, Paraffin-embedded Human Gastric carcinoma tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (ab111842)ab111842, at 1/500 dilution, staining FUZ in Formalin-fixed, Paraffin-embedded RT2 xenograft tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab111842 は 3 報の論文で使用されています。
- Wang S et al. Fuzzy Planar Cell Polarity Gene (FUZ) Promtes Cell Glycolysis, Migration, and Invasion in Non-small Cell Lung Cancer via the Phosphoinositide 3-Kinase/Protein Kinase B Pathway. J Cancer 13:2419-2429 (2022). PubMed: 35711836
- Zhang H et al. Development and Validation of an Immune-Related Signature for the Prediction of Recurrence Risk of Patients With Laryngeal Cancer. Front Oncol 11:683915 (2021). PubMed: 34976784
- Chen ZS et al. Planar cell polarity gene Fuz triggers apoptosis in neurodegenerative disease models. EMBO Rep 19:N/A (2018). PubMed: 30026307