Anti-FUT2 抗体 (ab177239)
Key features and details
- Goat polyclonal to FUT2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FUT2 antibody -
製品の詳細
Goat polyclonal to FUT2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla
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免疫原
Synthetic peptide corresponding to Human FUT2 aa 27-40 (internal sequence) (Cysteine residue). (near N terminus).
Sequence:HVQQRLAKIQAMWE
Database link: NP_000502.4 -
ポジティブ･コントロール
- Human liver lysate.
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特記事項
Reported variants represent identical protein: NP_001091107.1, NP_000502.4.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab177239 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177239の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. -
組織特異性
Small intestine, colon and lung. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 11 family. -
細胞内局在
Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi. - Information by UniProt
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参照データベース
- Entrez Gene: 468944 Chimpanzee
- Entrez Gene: 101148312 Gorilla
- Entrez Gene: 2524 Human
- Omim: 182100 Human
- SwissProt: O77485 Chimpanzee
- SwissProt: O77486 Gorilla
- SwissProt: Q10981 Human
- Unigene: 579928 Human
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別名
- 2)FT 2 antibody
- Alpha (1,2) fucosyltransferase antibody
- Alpha(1 antibody
see all
画像
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Western blot - Anti-FUT2 antibody (ab177239)Anti-FUT2 antibody (ab177239) at 1 µg/ml + Human liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab177239 は 1 報の論文で使用されています。
- Lili Q et al. Clostridium butyricum Induces the Production and Glycosylation of Mucins in HT-29 Cells. Front Cell Infect Microbiol 11:668766 (2021). PubMed: 34222040
