Anti-Furin 抗体 (ab28547)
Key features and details
- Rabbit polyclonal to Furin
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Furin antibody
Furin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Furin -
由来種
Rabbit -
特異性
ab28547 recognizes subtilisin-like serine protease furin. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Furin (N terminal).
(Peptide available asab41397) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody has been peptide-affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28547の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 88 kDa.
1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection. |
特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 88 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection. |
ターゲット情報
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機能
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
組織特異性
Seems to be expressed ubiquitously. -
配列類似性
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
ドメイン
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
翻訳後修飾
The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
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参照データベース
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- SwissProt: P23188 Mouse
- SwissProt: P23377 Rat
- Unigene: 5241 Mouse
- Unigene: 3220 Rat
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別名
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab28547 は 6 報の論文で使用されています。
- Braun E et al. Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins. Cell Rep 27:2092-2104.e10 (2019). PubMed: 31091448
- Jaaks P et al. The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells. Oncotarget 7:76743-76755 (2016). Human . PubMed: 27572312
- Jaaks P et al. The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion. PLoS One 11:e0161396 (2016). IHC . PubMed: 27548722
- Sathyamurthy M et al. Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7. Biotechnol Bioeng N/A:N/A (2014). WB . PubMed: 25219685
- Cekic M et al. Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury. Restor Neurol Neurosci 30:115-26 (2012). WB ; Rat . PubMed: 22232032
- Hajdin K et al. Furin targeted drug delivery for treatment of rhabdomyosarcoma in a mouse model. PLoS One 5:e10445 (2010). IHC-Fr ; Human . PubMed: 20454619