Anti-Furin 抗体 (ab177231)
Key features and details
- Goat polyclonal to Furin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Furin antibody
Furin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Furin -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Synthetic peptide corresponding to Human Furin aa 104-116 (internal sequence) (Cysteine residue).
Sequence:RTKRDVYQEPTDP
Database link: NP_002560.1 -
ポジティブ･コントロール
- HEK293 lysate overexpressing Human Furin with C-terminal MYC tag.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab177231 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177231の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa.
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa. A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
組織特異性
Seems to be expressed ubiquitously. -
配列類似性
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
ドメイン
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
翻訳後修飾
The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
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参照データベース
- Entrez Gene: 281374 Cow
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- Omim: 136950 Human
- SwissProt: Q28193 Cow
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
see all -
別名
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
画像
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Western blot - Anti-Furin antibody (ab177231)Lanes 1-2 : Anti-Furin antibody (ab177231) at 1 µg/ml
Lane 3 : anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate (in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag
Lane 2 : Mock-transfected HEK293 lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 87 kDaPrimary incubations were for 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab177231 は論文での使用が確認できていません。
