AB224722

Anti-FUNDC1 抗体

Name: Anti-FUNDC1 抗体 (ab224722) | Abcam
Brand: Abcam Limited
SKU: AB224722
Price: 73000 JPY
Availability: InStock

Anti-FUNDC1 antibody

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(36 Publications)

Rabbit Polyclonal FUNDC1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 36 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FUN14 domain-containing protein 1 aa 1-50.

別名を表示する

FUN14 domain-containing protein 1, FUNDC1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemical analysis of formalin fixed paraffin embedded human kidney tissue labelling FUNDC1 with ab224722 at a 1 : 250 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab224722 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemical analysis of formalin fixed paraffin embedded human small intestine tissue labelling FUNDC1 with ab224722 at a 1 : 250 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab224722 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemical analysis of formalin fixed paraffin embedded human colorectal cancer labelling FUNDC1 with ab224722 at a 1 : 250 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab224722 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human FUN14 domain-containing protein 1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8IVP5

特異性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purity >95%.

バッファー組成

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FUNDC1 also known as FUN14 domain-containing protein 1 is a protein involved in mitochondrial dynamics. It weighs approximately 15 kDa. FUNDC1 is primarily expressed in the mitochondrial outer membrane and shows high expression levels in tissues with high energy demands such as the heart and skeletal muscles. Its function is linked to the regulation of mitophagy a process that removes damaged mitochondria.

Biological function summary

FUNDC1 plays a role in the selective elimination of mitochondria through mitophagy. It acts as a receptor for damaged mitochondria binding to LC3 and enabling the autophagic machinery. FUNDC1 is not known to be part of a larger protein complex but it interacts with other mitophagy-related proteins such as PGAM5 and ULK1 to facilitate mitochondrial clearance.

Pathways

FUNDC1 participates in the mitophagy pathway and the broader mitochondrial quality control pathway. In the mitophagy pathway its interaction with proteins like LC3 facilitates the engulfment of dysfunctional mitochondria by autophagosomes. The relationship of FUNDC1 with ULK1 highlights its role in the initiation of the mitophagy signaling cascade ensuring mitochondrial integrity and cellular homeostasis.

FUNDC1 has associations with neurodegenerative diseases and cardiovascular disorders. Defects in the mitophagy process where FUNDC1 plays a significant role can contribute to the development of Parkinson's disease. Furthermore its connection to cardiovascular diseases involves interactions with BCL2L1 a protein that regulates apoptosis in heart tissue under stress conditions. Dysfunctional FUNDC1 activity may lead to the accumulation of damaged mitochondria contributing to cellular dysfunction observed in these diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Integral mitochondrial outer-membrane protein that mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs) (PubMed : 33972548). In turn, mediates angiogenesis and neoangiogenesis through interference with intracellular Ca(2+) communication and regulation of the vascular endothelial growth factor receptor KDR/VEGFR2 expression at both mRNA and protein levels (PubMed : 33972548). Also acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality and homeostasis, by interacting with and recruiting LC3 protein family to mitochondria (PubMed : 22267086, PubMed : 24671035, PubMed : 24746696, PubMed : 27653272). Mechanistically, recruits DRP1 at ER-mitochondria contact sites leading to DRP1 oligomerization and GTPase activity to facilitate mitochondrial fission during hypoxia (PubMed : 27145933, PubMed : 33978709). Additionally, plays a role in hepatic ferroptosis by interacting directly with glutathione peroxidase/GPX4 to facilitate its recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy (PubMed : 36828120).

See full target information FUN14 domain-containing protein 1

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文献 (36)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:22207 PubMed40593088

2025

Homocysteine promotes cardiomyocyte hypertrophy through inhibiting β-catenin/ FUNDC1 mediated mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Lei,Hengjing Hu,Huifang Tang,Hui Sun,Rui Liu,Yue Zhao

Alzheimer's research & therapy 17:47 PubMed39972387

2025

Cornuside alleviates cognitive impairments induced by Aβ through attenuating NLRP3-mediated neurotoxicity by promoting mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Fulin Zhou,Wenwen Lian,Xiaotang Yuan,Zexing Wang,Congyuan Xia,Yu Yan,Wenping Wang,Zhuohang Tong,Yungchi Cheng,Jiekun Xu,Jun He,Weiku Zhang

Acta physiologica (Oxford, England) 240:e14203 PubMed39023008

2024

A single bout of resistance exercise triggers mitophagy, potentially involving the ejection of mitochondria in human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Díaz-Castro,Mauro Tuñón-Suárez,Patricia Rivera,Javier Botella,Jorge Cancino,Ana María Figueroa,Juan Gutiérrez,Claudette Cantin,Louise Deldicque,Hermann Zbinden-Foncea,Joachim Nielsen,Carlos Henríquez-Olguín,Eugenia Morselli,Mauricio Castro-Sepúlveda

Open medicine (Warsaw, Poland) 19:20230810 PubMed38947217

2024

Methylation regulation for FUNDC1 stability in childhood leukemia was up-regulated and facilitates metastasis and reduces ferroptosis of leukemia through mitochondrial damage by FBXL2.

Applications

Unspecified application

Species

Unspecified reactive species

Sihai Tan,Yirong Ge,Jing Bi

Acta pharmacologica Sinica 45:1809-1820 PubMed38750074

2024

PHLDA1 contributes to hypoxic ischemic brain injury in neonatal rats via inhibiting FUNDC1-mediated mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Lu Jiang,Zu-Bin Zhang,Chen-Xi Feng,Chen-Jie Lin,Hui Yang,Lan-Lan Tan,Xin Ding,Li-Xiao Xu,Gen Li,Tao Pan,Zheng-Hong Qin,Bin Sun,Xing Feng,Mei Li

Bioactive materials 37:1-13 PubMed38515611

2024

Thread-structural microneedles loaded with engineered exosomes for annulus fibrosus repair by regulating mitophagy recovery and extracellular matrix homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojun Hu,Meng Zhu,Hongyuan Xing,Yucheng Xue,Jun Li,Zhan Wang,Zhou Zhu,Miaojie Fang,Zilong Li,Jianbin Xu,Yong He,Ning Zhang

Cell death and differentiation 31:335-347 PubMed38396150

2024

Exposure of the inner mitochondrial membrane triggers apoptotic mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tahnee L Saunders,Simon P Windley,Gediminas Gervinskas,Katherine R Balka,Caitlin Rowe,Rachael Lane,Maximilien Tailler,Thanh Ngoc Nguyen,Georg Ramm,Michael Lazarou,Dominic De Nardo,Benjamin T Kile,Kate McArthur

Redox biology 67:102906 PubMed37812880

2023

Neutrophil extracellular traps drive intestinal microvascular endothelial ferroptosis by impairing Fundc1-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chengnan Chu,Xinyu Wang,Chao Yang,Fang Chen,Lin Shi,Weiqi Xu,Kai Wang,Baochen Liu,Chenyang Wang,Dongping Sun,Weiwei Ding

Open medicine (Warsaw, Poland) 18:20230798 PubMed37808166

2023

Silencing USP19 alleviates cigarette smoke extract-induced mitochondrial dysfunction in BEAS-2B cells by targeting FUNDC1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjing You,Huijuan Wang,Qing Wang,Zongyang Yu,Zhongquan Zhao,Liying Zhuang,Shengyuan Zeng,Jinyang Zheng,Wen Wen

Journal of biochemical and molecular toxicology 37:e23503 PubMed37706594

2023

Poricoic acid A induces mitophagy to ameliorate podocyte injury in diabetic kidney disease via downregulating FUNDC1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwen Wu,Haohua Deng,Jiazhong Sun,Jun Tang,Xin Li,Yancheng Xu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FUNDC1 抗体

Anti-FUNDC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (AB224722)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (36)

Abcam product promise