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リコンビナントRabMAb

Anti-FUBP3 抗体 [EPR13174] (ab181025)

Submit a review Submit a question References (1)
Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)
  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (ab181025)
  • Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)
  • Anti-FUBP3 antibody [EPR13174] (ab181025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13174] to FUBP3
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-FUBP3 antibody [EPR13174]
    FUBP3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13174] to FUBP3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, HepG2 and MCF-7 cell lysate. Human liver and placenta tissue. HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181025の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IP
1/50.
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IP
1/50.
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.

  • 組織特異性

    Detected in a number of cell lines.

  • 配列類似性

    Contains 4 KH domains.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Far upstream element (FUSE) binding protein 3 antibody
      • Far upstream element binding protein 3 antibody
      • Far upstream element-binding protein 3 antibody
      • FBP3 antibody
      • FLJ25229 antibody
      • FUBP 3 antibody
      • FUBP3 antibody
      • FUBP3_HUMAN antibody
      • FUSE binding protein 3 antibody
      • FUSE-binding protein 3 antibody
      • OTTHUMP00000022369 antibody
      see all

    画像

    • Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025)
      Western blot - Anti-FUBP3 antibody [EPR13174] (ab181025)
      All lanes : Anti-FUBP3 antibody [EPR13174] (ab181025) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 62 kDa



      Blocking/ Dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)

      Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP3 antibody [EPR13174] (ab181025)

      Immunohistochemical analysis of paraffin embedded Human placenta tissue sections labeling FUBP3 using ab181025 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (ab181025)
      Immunocytochemistry/ Immunofluorescence - Anti-FUBP3 antibody [EPR13174] (ab181025)

      Immunofluorescent analysis of acetone fixed HeLa cells labeling FUBP3 using ab181025 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

    • Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)
      Immunoprecipitation - Anti-FUBP3 antibody [EPR13174] (ab181025)

      Lysate from HeLa cells was immunoprecipitated with ab181025 at a 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST

    • Anti-FUBP3 antibody [EPR13174] (ab181025)
      Anti-FUBP3 antibody [EPR13174] (ab181025)

    参考文献 (1)

    ab181025 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181025 は 1 報の論文で使用されています。

    • Yao Q  et al. Lnc-EST12, which is negatively regulated by mycobacterial EST12, suppresses antimycobacterial innate immunity through its interaction with FUBP3. Cell Mol Immunol 19:883-897 (2022). PubMed: 35637281

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