Anti-FTSJD2 抗体 (ab70386)
Key features and details
- Rabbit polyclonal to FTSJD2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FTSJD2 antibody
FTSJD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FTSJD2 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
A region between residues 1 and 50 of human FTSJD2.
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ポジティブ･コントロール
- WB: HeLa, HEK239T, TCMK-1, Jurkat and NIH3T3 whole cell lysates. IP: 293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70386の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 1 mg/ml.
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| WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa).
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| 特記事項 |
|---|
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IP
Use a concentration of 1 mg/ml. |
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WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa). |
ターゲット情報
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機能
S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway. -
配列類似性
Contains 1 G-patch domain.
Contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain.
Contains 1 WW domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23070 Human
- Entrez Gene: 74157 Mouse
- Entrez Gene: 100172922 Orangutan
- SwissProt: Q8N1G2 Human
- SwissProt: Q9DBC3 Mouse
- SwissProt: Q5R981 Orangutan
- Unigene: 520102 Human
- Unigene: 390979 Mouse
see all -
別名
- Cap methyltransferase 1 antibody
- Cap-specific mRNA (nucleoside-2''-O-)-methyltransferase 1 antibody
- Cap1 2''O-ribose methyltransferase 1 antibody
see all
画像
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Western blot - Anti-FTSJD2 antibody (ab70386)All lanes : Anti-FTSJD2 antibody (ab70386) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 96 kDa -
Immunoprecipitation - Anti-FTSJD2 antibody (ab70386)Detection of human FTSJD2 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.
Lane 1: Rabbit polyclonal to FTSJD2 (ab70742) at 6 µg per reaction
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 30 seconds. ab70742 was used at 1 ug/mL for Western blot.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70386 は 1 報の論文で使用されています。
- You AB et al. CMTR1 promotes colorectal cancer cell growth and immune evasion by transcriptionally regulating STAT3. Cell Death Dis 14:245 (2023). PubMed: 37024465