Anti-FRK 抗体 (ab64914)
Key features and details
- Rabbit polyclonal to FRK
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FRK antibody
FRK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FRK -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide derived from an internal sequence of Human FRK
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ポジティブ･コントロール
- Human breast carcinoma tissue, HUVEC cells and extracts from Jurkat cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64914の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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| ICC/IF |
1/500 - 1/1000.
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| IHC-P | (1) |
1/50 - 1/100.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
|
ICC/IF
1/500 - 1/1000. |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor. -
組織特異性
Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2444 Human
- Entrez Gene: 14302 Mouse
- Entrez Gene: 79209 Rat
- Omim: 606573 Human
- SwissProt: P42685 Human
- SwissProt: Q922K9 Mouse
- SwissProt: Q62662 Rat
- Unigene: 89426 Human
see all -
別名
- Beta cell Src homology tyrosine kinase antibody
- BSK antibody
- BSK/IYK antibody
see all
画像
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Western blot - Anti-FRK antibody (ab64914)All lanes : Anti-FRK antibody (ab64914) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 56 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRK antibody (ab64914)ab64914, at a 1/50 dilution, staining Human FRK in paraffin embedded breast carcinoma tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-FRK antibody (ab64914)ab64914, at a 1/500 dilution, staining Human FRK in HUVEC cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab64914 は 2 報の論文で使用されています。
- Yu XZ et al. TRIM44 facilitates ovarian cancer proliferation, migration, and invasion by inhibiting FRK. Neoplasma 68:751-759 (2021). PubMed: 34034495
- Yumnam S et al. Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array. Mol Med Rep 16:5386-5392 (2017). PubMed: 28849065