Anti-Frizzled 5 抗体 (ab14475)
Key features and details
- Rabbit polyclonal to Frizzled 5
- Suitable for: ICC/IF, IHC-FoFr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Frizzled 5 antibody
Frizzled 5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Frizzled 5 -
由来種
Rabbit -
特異性
Recognizes human Fz5. -
アプリケーション
適用あり: ICC/IF, IHC-FoFr, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Frizzled 5 aa 550-650 (C terminal) conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol, 0.609% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14475の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration. PubMed: 20530549
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20530549
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 20530549 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20530549 |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. |
ターゲット情報
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機能
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway. -
配列類似性
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
ドメイン
The PDZ-binding motif mediates interaction with GOPC.
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
細胞内局在
Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi. - Information by UniProt
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参照データベース
- Entrez Gene: 7855 Human
- Entrez Gene: 14367 Mouse
- Entrez Gene: 317674 Rat
- Omim: 601723 Human
- SwissProt: Q13467 Human
- SwissProt: Q9EQD0 Mouse
- SwissProt: Q8CHL0 Rat
- Unigene: 17631 Human
see all -
別名
- C2orf31 antibody
- DKFZP434E2135 antibody
- Frizzled 5 seven transmembrane spanning receptor antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab14475 は 6 報の論文で使用されています。
- Zhang L et al. Silencing of Long Noncoding RNA SOX21-AS1 Relieves Neuronal Oxidative Stress Injury in Mice with Alzheimer's Disease by Upregulating FZD3/5 via the Wnt Signaling Pathway. Mol Neurobiol 56:3522-3537 (2019). PubMed: 30143969
- Skaria T et al. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells. Cell Adh Migr 11:24-38 (2017). PubMed: 27159116
- Ma M et al. Wnt signaling in form deprivation myopia of the mice retina. PLoS One 9:e91086 (2014). WB ; Mouse . PubMed: 24755605
- Ye Z et al. Wnt5a uses CD146 as a receptor to regulate cell motility and convergent extension. Nat Commun 4:2803 (2013). PubMed: 24335906
- Sahores M et al. Frizzled-5, a receptor for the synaptic organizer Wnt7a, regulates activity-mediated synaptogenesis. Development 137:2215-25 (2010). ICC/IF, IHC-Fr ; Mouse, Rat . PubMed: 20530549
- Pereira C et al. Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10. Arterioscler Thromb Vasc Biol 28:504-10 (2008). PubMed: 18174455