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リコンビナントRabMAb

Anti-Fragilis 抗体 [EPR5242] (ab109429)

Reviews (1)Q&A (1)References (21)
Western blot - Anti-Fragilis antibody [EPR5242] (ab109429)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fragilis antibody [EPR5242] (ab109429)
  • Western blot - Anti-Fragilis antibody [EPR5242] (ab109429)
  • Anti-Fragilis antibody [EPR5242] (ab109429)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5242] to Fragilis
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Fragilis antibody [EPR5242]
    Fragilis 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5242] to Fragilis

  • 由来種

    Rabbit

  • 特異性

    Predicted cross-reactivity with IFITM2 (93% homology) but this has not been tested.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa whole cell lysate (PMID: 21177806), Recombinant Human Fragilis protein. IHC-P: Human kidney tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR5242

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109429の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication.

  • 配列類似性

    Belongs to the CD225 family.

  • 翻訳後修飾

    Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 1 8U antibody
      • Fragilis antibody
      • IFITM3 antibody
      • IFM3_HUMAN antibody
      • Interferon induced transmembrane protein 3 (1 8U) antibody
      • Interferon Induced Transmembrane Protein 3 antibody
      • Interferon inducible antibody
      • Interferon inducible protein 1 8U antibody
      • Interferon Inducible Protein 15 antibody
      • Interferon Inducible Protein Homolog antibody
      • Interferon-induced transmembrane protein 3 antibody
      • Interferon-inducible protein 1-8U antibody
      • IP15 antibody
      see all

    画像

    • Western blot - Anti-Fragilis antibody [EPR5242] (ab109429)
      Western blot - Anti-Fragilis antibody [EPR5242] (ab109429)
      All lanes : Anti-Fragilis antibody [EPR5242] (ab109429) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : HAP1 (Human chronic myelogenous leukemia near-haploid cell line) whole cell lysate
      Lane 4 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 15 kDa
      Observed band size: 15 kDa


      Exposure time: 1 second


      Lane 1: Positive control - Hela (PMID: 21177806)

      Lanes 2-4: Negative control - 293T and Jurkat (PMID: 21177806) HAP1(PMID: 29079573).

      Blocking buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fragilis antibody [EPR5242] (ab109429)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fragilis antibody [EPR5242] (ab109429)

      Immunohistochemical analysis of Fragilis in paraffin-embedded Human kidney tissue using ab109429 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Fragilis antibody [EPR5242] (ab109429)
      Western blot - Anti-Fragilis antibody [EPR5242] (ab109429)
      Anti-Fragilis antibody [EPR5242] (ab109429) at 1/1000 dilution + Recombinant Human Fragilis protein (ab92921) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 15 kDa


      Exposure time: 10 seconds
    • Anti-Fragilis antibody [EPR5242] (ab109429)
      Anti-Fragilis antibody [EPR5242] (ab109429)

    参考文献 (21)

    ab109429 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109429 は 21 報の論文で使用されています。

    • Degrelle SA  et al. IFITM1 inhibits trophoblast invasion and is induced in placentas associated with IFN-mediated pregnancy diseases. iScience 26:107147 (2023). PubMed: 37434700
    • Verma S  et al. IFITM1 and IFITM3 Proteins Inhibit the Infectivity of Progeny HIV-1 without Disrupting Envelope Glycoprotein Clusters. Viruses 15:N/A (2023). PubMed: 38140631
    • Liang G  et al. Identification of differentially expressed and methylated genes and construction of a co-expression network in age-related macular degeneration. Ann Transl Med 10:223 (2022). PubMed: 35280389
    • Rahman K  et al. Cholesterol Binds the Amphipathic Helix of IFITM3 and Regulates Antiviral Activity. J Mol Biol 434:167759 (2022). PubMed: 35872070
    • Shi G  et al. Rapalogs downmodulate intrinsic immunity and promote cell entry of SARS-CoV-2. bioRxiv N/A:N/A (2021). PubMed: 33880473
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Fragilis antibody [EPR5242]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (Blastocyst Day7)
    Permeabilization
    Yes - 0,2% Triton X
    Specification
    Blastocyst Day7
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Irene Röttinger

    Verified customer

    投稿 Nov 30 2016

    Question

    Possible cross reactivity with IFITM2. Immunogen is 92% homologous.

    Read More
    Answer

    Thank you for contacting Abcam.



    The immunogen for cat.# ab109429 has a 93% homology with IFITM2. However, we have not tested any potential cross-reactivity with IFITM2. I would predict cross-reactivity, but it is not guaranteed without testing. I am asking our datasheets team to look into this and make any necessary adjustments to the datasheet for this product.



    Please let me know if you have any questions.

    Read More

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