Anti-Fragilis 抗体 (ab15592)
Key features and details
- Rabbit polyclonal to Fragilis
- Suitable for: IP, ICC/IF, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Fragilis antibody
Fragilis 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Fragilis -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IP, ICC/IF, IHC-Fr, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: mES cells. IHC-Fr: E12.5 mouse embryos. WB: Mouse embryonic stem cell, Mouse embryonic germ cell lysates. IP: E14Tg2a whole cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15592の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use a concentration of 1 - 10 µg/ml.
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IHC-Fr |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
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特記事項 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 10 µg/ml. |
IHC-Fr
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). |
ターゲット情報
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機能
IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. -
配列類似性
Belongs to the CD225 family. -
翻訳後修飾
Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10410 Human
- Entrez Gene: 66141 Mouse
- Entrez Gene: 361673 Rat
- Omim: 605579 Human
- SwissProt: Q01628 Human
- SwissProt: Q9CQW9 Mouse
- SwissProt: P26376 Rat
- Unigene: 374650 Human
see all -
別名
- 1 8U antibody
- Fragilis antibody
- IFITM3 antibody
see all
画像
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ab15592 staining Fragilis in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab15592 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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All lanes : Anti-Fragilis antibody (ab15592) at 1 µg/ml
Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa -
IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).
ab15592 (anti-Fragilis) = red
Oct4 = green
DAPI (DNA) = blueThe dilution of ab15592 used was 10 ug/ml.
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Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 13kDa: Fragilis - Primordial Germ Cell Marker.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab15592 は 39 報の論文で使用されています。
- Yang J et al. A novel co-culture model for investigation of the effects of LPS-induced macrophage-derived cytokines on brain endothelial cells. PLoS One 18:e0288497 (2023). PubMed: 37440496
- Li N et al. Interferon-induced transmembrane protein 3 in the hippocampus: a potential novel target for the therapeutic effects of recombinant human brain natriuretic peptide on sepsis-associated encephalopathy. Front Mol Neurosci 16:1182005 (2023). PubMed: 37602193
- Yu X et al. Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro. Stem Cell Rev Rep 19:1067-1081 (2023). PubMed: 36735215
- Noval MG et al. MAVS signaling is required for preventing persistent chikungunya heart infection and chronic vascular tissue inflammation. Nat Commun 14:4668 (2023). PubMed: 37537212
- Nahaboo W et al. Keratin filaments mediate the expansion of extra-embryonic membranes in the post-gastrulation mouse embryo. EMBO J 41:e108747 (2022). PubMed: 35266581