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    products/primary-antibodies/fprl1rfp-antibody-ab63022.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-FPRL1/RFP 抗体 (ab63022)

  • Datasheet
Submit a review Q&A (2)References (6)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Flow Cytometry - Anti-FPRL1/RFP antibody (ab63022)
  • Western blot - Anti-FPRL1/RFP antibody (ab63022)
  • Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody (ab63022)

Key features and details

  • Rabbit polyclonal to FPRL1/RFP
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human FPRL1/RFP protein (ab114357)
一次抗体
Product image
Anti-RFP antibody (ab152123)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-FPRL1/RFP antibody
    FPRL1/RFP 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to FPRL1/RFP
  • 由来種

    Rabbit
  • 特異性

    This antibody recognizes human FPRL-1.
  • アプリケーション

    適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human FPRL1/RFP conjugated to keyhole limpet haemocyanin.

  • ポジティブ・コントロール

    • HL60 cells and extracts from HL60 cells
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HL-60 whole cell lysate (ab7914)
  • Recombinant Protein

    • Recombinant Human FPRL1/RFP protein (ab114357)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab63022の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).
Flow Cyt
1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF
1/500.
特記事項
WB
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).
Flow Cyt
1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF
1/500.

ターゲット情報

  • 機能

    Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
  • 組織特異性

    Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在

    Cell membrane.
  • Target information above from: UniProt accession P25090 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2358 Human
    • Omim: 136538 Human
    • SwissProt: P25090 Human
    • Unigene: 99855 Human
    • 別名

      • ALXR antibody
      • FMLP-R-I antibody
      • FMLP-R-II antibody
      • FMLP-related receptor I antibody
      • FMLPX antibody
      • Formyl peptide receptor 2 antibody
      • Formyl peptide receptor related antibody
      • Formyl peptide receptor-like 1 antibody
      • FPR2 antibody
      • FPR2_HUMAN antibody
      • FPR2A antibody
      • FPRH1 antibody
      • FPRH2 antibody
      • FPRL1 antibody
      • HM63 antibody
      • Lipoxin A4 receptor antibody
      • LXA4 receptor antibody
      • LXA4R antibody
      • N-formyl peptide receptor 2 antibody
      • RFP antibody
      see all

    画像

    • Flow Cytometry - Anti-FPRL1/RFP antibody (ab63022)
      Flow Cytometry - Anti-FPRL1/RFP antibody (ab63022)
      Flow cytometric analysis of HL60 cells using ab63022 at 1:100 (red) compared to a non-specific negative control antibody (black).
    • Western blot - Anti-FPRL1/RFP antibody (ab63022)
      Western blot - Anti-FPRL1/RFP antibody (ab63022)
      Anti-FPRL1/RFP antibody (ab63022) at 1/5000 dilution + Extracts from HL60 cells.

      Predicted band size: 39 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody (ab63022)
      Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody (ab63022)
      Immunofluorescent analysis of HL60 cells using ab63022 (green) at 1:500. Nucleus have bee labeled with PI (red).

    プロトコール

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (6)

    ab63022 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab63022 は 6 報の論文で使用されています。

    • Li S  et al. Suppression of FPR2 expression inhibits inflammation in preeclampsia by improving the biological functions of trophoblast via NF-κB pathway. J Assist Reprod Genet 39:239-250 (2022). PubMed: 35018584
    • Lannan KL  et al. Maresin 1 induces a novel pro-resolving phenotype in human platelets. J Thromb Haemost 15:802-813 (2017). PubMed: 28079976
    • Yu N  et al. Serum amyloid A, an acute phase protein, stimulates proliferative and proinflammatory responses of keratinocytes. Cell Prolif 50:N/A (2017). PubMed: 27910163
    • Hsiao HM  et al. Resolvin D1 Reduces Emphysema and Chronic Inflammation. Am J Pathol 185:3189-201 (2015). PubMed: 26468975
    • Wood MP  et al. The HIV-1 gp41 ectodomain is cleaved by matriptase to produce a chemotactic peptide that acts through FPR2. Immunology 142:474-83 (2014). PubMed: 24617769
    • Gori I  et al. Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4). Fertil Steril 99:1965-73.e2 (2013). PubMed: 23472950

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    I was interested in purchasing the ab63022 Anti-FPRL1 antibody. I see it has been tested with facs before. However would you know if the image provide was investigating FPRL1 expression on the surface of cells? I would require this anitbody to prob for surface expression of this receptor, would it be suitable for this purpose? Also could i ask for you to recommend an iso type control and secondary conjugate link.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    Thank you for your inquiry. Yes, this antibody will detect the 2nd extracellular loop of FPRL1, on the surface of cells. As for a suitable secondary antibody, we have a good goat polyclonal secondary antibody to rabbit IgG that is FITC-conjugated and tested in FC, ab97050. https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-h-l-fitc-ab97050.html A good isotype control would be Rabbit IgG isotype control, ab37415. https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html I hope this information helps.  Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 19 2011

    Question

    I am interested at your anti-FPRL1 antibodies, ab63022 and ab63023. They are indicated to react with human specie, but what we are doing is mouse sample. I would be really appreciated if you could tell whether it works also on mouse samples. Thank you very much.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 20 2011

    Answer

    Thank you for your inquiry. We haven't tested ab63022 or ab63023 in mouse so we do not know if these antibodies will work in mouse. I've BLASTed the immunogen sequences against mouse and the results are below: Ab63022 was immunized against the 2nd extracelluar domain of FPRL-1. This sequence is only 50% homologous with mouse. Ab63023 was immunized against the C-terminal sequence of FPRL-1. This sequence is only 61% homologous with mouse. We typically say that anything with > 85% sequence homology would be expected to cross-react, therefore the chances are a bit slim with these antibodies. However, we do have a testing discount program which you could participate in to test ab63023 (the one with a greater homology) with mouse. To our knowledge, ab63023 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab63023 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab63023 in mouse. I will then send a discount code. This code must be issued before purchasing ab63023 so please wait for my reply before ordering. 2. Purchase ab63023 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab63023 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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