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リコンビナントRabMAb

Anti-FPGS 抗体 [EPR11064] (ab184564)

Submit a review Submit a question References (2)
Western blot - Anti-FPGS antibody [EPR11064] (ab184564)
  • Western blot - Anti-FPGS antibody [EPR11064] (ab184564)
  • Flow Cytometry (Intracellular) - Anti-FPGS antibody [EPR11064] (ab184564)
  • Anti-FPGS antibody [EPR11064] (ab184564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11064] to FPGS
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-FPGS antibody [EPR11064]
    FPGS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11064] to FPGS

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • JAR, HeLa and K562 cell lysates. HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184564の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
特記事項
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

ターゲット情報

  • 機能

    Conversion of folates to polyglutamate derivatives. This allows tissues to concentrate folate at higher levels than in plasma.

  • パスウェイ

    Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.

  • 配列類似性

    Belongs to the folylpolyglutamate synthase family.

  • 細胞内局在

    Mitochondrion. Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EC 6.3.2.17 antibody
      • FOLC antibody
      • FOLC_HUMAN antibody
      • Folylpoly gamma glutamate synthetase antibody
      • Folylpoly-gamma-glutamate synthetase antibody
      • Folylpolyglutamate synthase antibody
      • Folylpolyglutamate synthase mitochondrial antibody
      • FPGS antibody
      • mitochondrial antibody
      • OTTHUMP00000022201 antibody
      • OTTHUMP00000022202 antibody
      • Tetrahydrofolate synthase antibody
      • Tetrahydrofolylpolyglutamate synthase antibody
      see all

    画像

    • Western blot - Anti-FPGS antibody [EPR11064] (ab184564)
      Western blot - Anti-FPGS antibody [EPR11064] (ab184564)
      Anti-FPGS antibody [EPR11064] (ab184564) at 1/50000 dilution + K562 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Western blot - Anti-FPGS antibody [EPR11064] (ab184564)
      Western blot - Anti-FPGS antibody [EPR11064] (ab184564)
      All lanes : Anti-FPGS antibody [EPR11064] (ab184564) at 1/10000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Flow Cytometry (Intracellular) - Anti-FPGS antibody [EPR11064] (ab184564)
      Flow Cytometry (Intracellular) - Anti-FPGS antibody [EPR11064] (ab184564)

      Intracellular Flow Cytometry analysis ofFPGS expression in paraformaldehyde-fixed HeLa cells using ab184564 at 1/60 dilution (red) and a rabbit IgG as negative control (green). Secondary antibody: Goat anti-rabbit IgG (FITC). 

       

       

    • Anti-FPGS antibody [EPR11064] (ab184564)
      Anti-FPGS antibody [EPR11064] (ab184564)

    参考文献 (2)

    ab184564 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184564 は 2 報の論文で使用されています。

    • Oiwa K  et al. Characterization of newly established Pralatrexate-resistant cell lines and the mechanisms of resistance. BMC Cancer 21:879 (2021). PubMed: 34332580
    • Srivastava AC  et al. Elimination of human folypolyglutamate synthetase alters programming and plasticity of somatic cells. FASEB J 33:13747-13761 (2019). PubMed: 31585510

    レビューと Q&A

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