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Anti-FOXP3 抗体 [2A11G9] (ab36607)

Submit a review Q&A (1)References (5)
Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)
  • Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)

Key features and details

  • Mouse monoclonal [2A11G9] to FOXP3
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-FOXP3 antibody [2A11G9]
    FOXP3 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2A11G9] to FOXP3

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, WB, Flow Cytmore details
    適用なし: IHC-Fr or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to FOXP3.
    Database link: Q9BZS1-1

  • ポジティブ･コントロール

    • HEK293 cell extract.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab36607の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/200 - 1/1000.
WB
1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
1/200 - 1/1000.
WB
1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for IHC-Fr or IHC-P.

ターゲット情報

  • 機能

    Probable transcription factor. Plays a critical role in the control of immune response.

  • 関連疾患

    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.

  • 配列類似性

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    画像

    • Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)
      Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)
      Overlay histogram showing Jurkat cells stained with ab36607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)
      Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)
      All lanes : Anti-FOXP3 antibody [2A11G9] (ab36607) at 1/1000 dilution

      Lane 1 : Human truncated Foxp3 recombinant protein
      Lane 2 : HEK293 cell extract

      Lysates/proteins at 2 µg/ml per lane.

      Predicted band size: 47 kDa
      Observed band size: ~50-53 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)
      Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)

      ab36607 at 1/1000 dilution staining FOXP3 in human PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary antibody show green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 Phalloidin.

    参考文献 (5)

    ab36607 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab36607 は 5 報の論文で使用されています。

    • Park TY  et al. Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases. Sci Rep 9:15559 (2019). PubMed: 31664129
    • Su H  et al. Mycobacterium tuberculosis PPE60 antigen drives Th1/Th17 responses via Toll-like receptor 2-dependent maturation of dendritic cells. J Biol Chem 293:10287-10302 (2018). PubMed: 29739853
    • Ji H  et al. Sex-specific T-cell regulation of angiotensin II-dependent hypertension. Hypertension 64:573-82 (2014). PubMed: 24935938
    • Imam S  et al. Diabetes-associated dry eye syndrome in a new humanized transgenic model of type 1 diabetes. Mol Vis 19:1259-67 (2013). ICC/IF ; Mouse . PubMed: 23805032
    • Singh TP  et al. 8-methoxypsoralen plus ultraviolet A therapy acts via inhibition of the IL-23/Th17 axis and induction of Foxp3+ regulatory T cells involving CTLA4 signaling in a psoriasis-like skin disorder. J Immunol 184:7257-67 (2010). PubMed: 20488788

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Product Anti-FOXP3 antibody [2A11G9] (ab36607) is a mouse monoclonal Ab, and we are working with mouse tissue sections – is this Ab suitable for IHC staining with mouse tissue?

    Can you recommend a product or protocol to achieve clean mouse-on-mouse staining in IHC?

    Read More
    Answer

    Unfortunately, ab36607 is not recommended for IHC applications (IHC-Fr or IHC-P).

    For Mouse-on-Mouse (MOM) staining with other mouse-host antibodies, we can recommend the following IHC detection kit: Mouse on Mouse Polymer IHC Kit (ab127055).

    Alternatively, our suggested MOM Staining Protocol can be found here: MOM Staining Protocol.

    Read More

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