Anti-FOXP2 抗体 [73A/8] (ab172320)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody [73A/8] (ab172320)](https://www.abcam.co.jp/ps/products/172/ab172320/Images/ab172320-218053-anti-foxp2-antibody-73a-8-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody [73A/8] (ab172320)")
Key features and details
- Mouse monoclonal [73A/8] to FOXP2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-FOXP2 antibody [73A/8]
FOXP2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [73A/8] to FOXP2 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human FOXP2.
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ポジティブ･コントロール
- WB: HeLa and HEK293 whole cell lysates. IHC-P: FFPE human lung tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
73A/8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172320の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 73,75 kDa (predicted molecular weight: 80 kDa).
Abcam recommends blocking with 3% milk. |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 73,75 kDa (predicted molecular weight: 80 kDa). Abcam recommends blocking with 3% milk. |
ターゲット情報
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機能
Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language. -
組織特異性
Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung. -
関連疾患
Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2). -
配列類似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
発生段階
Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber. -
ドメイン
The leucine-zipper is required for dimerization and transcriptional repression. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 93986 Human
- Omim: 605317 Human
- SwissProt: O15409 Human
- Unigene: 282787 Human
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別名
- CAG repeat protein 44 antibody
- CAGH44 antibody
- DKFZp686H1726 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody [73A/8] (ab172320)
IHC image of FOXP2 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab172320, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-FOXP2 antibody [73A/8] (ab172320)](https://www.abcam.co.jp/ps/products/172/ab172320/Images/ab172320-232479-anti-foxp2-antibody-73a-8-western-blot.jpg)
Western blot - Anti-FOXP2 antibody [73A/8] (ab172320)Lane 1 : Anti-FOXP2 antibody [73A/8] (ab172320) at 5 µg/ml (blocked with 3% milk)
Lane 2 : Anti-FOXP2 antibody [73A/8] (ab172320) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 73,75 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab172320 は論文での使用が確認できていません。