Anti-FOXP1 抗体 [JC12] (ab32010)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.co.jp/ps/products/32/ab32010/Images/ab32010-274940-anti-foxp1-antibody-jc12-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)")
Key features and details
- Mouse monoclonal [JC12] to FOXP1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-FOXP1 antibody [JC12]
FOXP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [JC12] to FOXP1 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Zebra finch
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免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: BCBL1 human cell line or Mutul human cell line. IHC-P: Human Hodgkin's lymphoma and human colon tissues.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 6.97% L-Arginine, PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
JC12 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32010の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (3) |
1/500. Predicted molecular weight: 75 kDa.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/500. Predicted molecular weight: 75 kDa. |
ターゲット情報
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機能
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
関連疾患
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
配列類似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
ドメイン
The leucine-zipper is required for dimerization and transcriptional repression. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Omim: 605515 Human
- SwissProt: Q9H334 Human
- SwissProt: P58462 Mouse
- Unigene: 431498 Human
- Unigene: 234965 Mouse
- Unigene: 485266 Mouse
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別名
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)
IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.co.jp/ps/products/32/ab32010/Images/ab32010-43628-anti-foxp1-antibody-jc12-western-blot.jpg)
Western blot - Anti-FOXP1 antibody [JC12] (ab32010)All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HEK293 Whole Cell Lysate Overexpressing FOXP1
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
Predicted band size: 75 kDa
Observed band size: 100,80 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.co.jp/ps/products/32/ab32010/Images/ab32010-274942-anti-foxp1-antibody-jc12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma (CD30+)*, showing positive staining in the mantle zone and negative staining in the Hodgkin's cells and germinal centre. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.co.jp/ps/products/32/ab32010/Images/ab32010-43629-anti-foxp1-antibody-jc12-western-blot.jpg)
Western blot - Anti-FOXP1 antibody [JC12] (ab32010)All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 1/500 dilution
Lane 1 : BCBL1 human cell line
Lane 2 : Mutul human cell line
Predicted band size: 75 kDa
Observed band size: 60,65,80 kDa why is the actual band size different from the predicted?
This antibody recognizes a long form and two short forms of human FOXP1 as described in Blood. 2008. Mar 1; 111(5): 2816-24.
Image kindly provided by H. Hu.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (42)
ab32010 は 42 報の論文で使用されています。
- Mansky RH et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease. Cell Rep 42:112198 (2023). PubMed: 36867535
- Sanders B et al. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants. Nat Commun 13:27 (2022). PubMed: 35031607
- Neyroud D et al. FoxP1 is a transcriptional repressor associated with cancer cachexia that induces skeletal muscle wasting and weakness. J Cachexia Sarcopenia Muscle 12:421-442 (2021). PubMed: 33527776
- Garcia-Oscos F et al. Autism-linked gene FoxP1 selectively regulates the cultural transmission of learned vocalizations. Sci Adv 7:N/A (2021). PubMed: 33536209
- Anderson AG et al. Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development. Cell Rep 30:3051-3066.e7 (2020). PubMed: 32130906