Anti-FOXP1 抗体 (ab16645)
Key features and details
- Rabbit polyclonal to FOXP1
- Suitable for: IHC-FoFr, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FOXP1 antibody
FOXP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXP1 -
由来種
Rabbit -
特異性
Replenishment batches of our polyclonal antibody, ab16645 are tested in IHC-P. Previous batches were additionally validated in IHC-FoFr and WB. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab134055.
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アプリケーション
適用あり: IHC-FoFr, IHC-P, WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Chicken, Dog, Human, Xenopus laevis
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-p: Mouse brain
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16645の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr |
Use at an assay dependent concentration.
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| IHC-P | (4) |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (7) |
1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details). |
| 特記事項 |
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IHC-FoFr
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details). |
ターゲット情報
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機能
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
関連疾患
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
配列類似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
ドメイン
The leucine-zipper is required for dimerization and transcriptional repression. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 416092 Chicken
- Entrez Gene: 484692 Dog
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 297480 Rat
- Entrez Gene: 496386 Xenopus laevis
- Entrez Gene: 779033 Xenopus laevis
- Omim: 605515 Human
see all -
別名
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody (ab16645)
Paraffin-embedded mouse brain tissue stained for FOXP1 using ab16645 in immunohistochemical analysis.
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Western blot - Anti-FOXP1 antibody (ab16645)All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution
Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 65,82 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity) -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-FOXP1 antibody (ab16645)This image is courtesy of Shunyou Wang, Ph.D.ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (103)
ab16645 は 103 報の論文で使用されています。
- Limone F et al. Efficient generation of lower induced motor neurons by coupling Ngn2 expression with developmental cues. Cell Rep 42:111896 (2023). PubMed: 36596304
- Liau ES et al. Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons. Nat Commun 14:46 (2023). PubMed: 36596814
- Zhang M et al. Neuronal Histone Methyltransferase EZH2 Regulates Neuronal Morphogenesis, Synaptic Plasticity, and Cognitive Behavior in Mice. Neurosci Bull 39:1512-1532 (2023). PubMed: 37326884
- Ismailova A et al. Identification of a forkhead box protein transcriptional network induced in human neutrophils in response to inflammatory stimuli. Front Immunol 14:1123344 (2023). PubMed: 36756115
- Heim F et al. Effects of Cortical FoxP1 Knockdowns on Learned Song Preference in Female Zebra Finches. eNeuro 10:N/A (2023). PubMed: 36931727