AB249138

Anti-Foxo6 (phospho S184) 抗体 [EPR6863] - BSA and Azide free

Anti-Foxo6 (phospho S184) antibody [EPR6863] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal FOXO6 phospho S184 antibody. Carrier free. Suitable for WB and reacts with Rat samples.

別名を表示する

Forkhead box protein O6, FOXO6

1 Images

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Western blot - Anti-Foxo6 (phospho S184) antibody [EPR6863] - BSA and Azide free (AB249138)

This data was developed using ab154832, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Foxo6 (phospho S184) antibody [EPR6863] (<a href='/products/primary-antibodies/foxo6-phospho-s184-antibody-epr6863-ab154832'>ab154832</a>) at 1/1000 dilution

Lane 1:

Rat brain lysate, untreated at 10 µg

Lane 2:

Rat brain lysate, treated with AP at 10 µg

Predicted band size: 61 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6863

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Rat

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

ab249138 is the carrier-free version of ab154832.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Foxo6 protein also known as Forkhead box O6 is part of the Forkhead family of transcription factors characterized by a distinct forkhead DNA-binding domain. It has a molecular weight of approximately 68 kDa. Foxo6 is primarily expressed in the brain with significant presence in the hippocampus which plays an essential role in cognitive functions. Functionally Foxo6 regulates gene expression by binding to specific DNA sequences influencing various cellular processes including apoptosis metabolism and oxidative stress response.

Biological function summary

The regulatory activity of Foxo6 is important for maintaining neural integrity and proper brain function. It does not work as part of a larger complex but influences the expression of genes involved in cell cycle arrest longevity and stress resistance. Researchers have observed Foxo6 activity to affect synaptic function and plasticity in neurons which are critical for learning and memory processes. Its role in transcriptional regulation highlights its significance in managing cellular homeostasis and response to environmental changes.

Pathways

Foxo6 acts within the insulin signaling and PI3K/AKT pathways which are central to regulating growth proliferation and survival. Through these pathways Foxo6 interacts with proteins like AKT1 and 14-3-3 which modulate its activity by nuclear-cytoplasmic shuttling or degradation. The balance of Foxo6 activity within these pathways influences how cells respond to insulin and manage oxidative stress linking it to metabolic and stress response processes.

Aberrant Foxo6 activity links to neurodegenerative disorders and metabolic syndromes. In the context of Alzheimer's disease Foxo6 dysfunction may contribute to impaired cognition and synaptic deficits as its regulatory influence is important for neural health. Additionally altered Foxo6 signaling can affect insulin sensitivity relevant in conditions like type 2 diabetes as it connects through the insulin signaling pathway. These connections position Foxo6 as a potential therapeutic target for ailments associated with metabolic and cognitive impairments.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional activator.

See full target information FOXO6 pS184

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Foxo6 (phospho S184) 抗体 [EPR6863] - BSA and Azide free

Anti-Foxo6 (phospho S184) antibody [EPR6863] - BSA and Azide free

Western blot - Anti-Foxo6 (phospho S184) antibody [EPR6863] - BSA and Azide free (AB249138)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise