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Anti-FOXO4/AFX (phospho T451) 抗体 (ab79188)

Reviews (1)Q&A (2)References (1)
Western blot - Anti-FOXO4/AFX (phospho T451) antibody (ab79188)

    Key features and details

    • Rabbit polyclonal to FOXO4/AFX (phospho T451)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-FOXO4/AFX (phospho T451) antibody
      FOXO4/AFX 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to FOXO4/AFX (phospho T451)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse

    • 免疫原

      Synthetic peptide corresponding to Human FOXO4/AFX (phospho T451).

    • ポジティブ･コントロール

      • Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins).

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      ab79188 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab79188の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    1/500 - 1/1000. Predicted molecular weight: 54 kDa.
    特記事項
    WB
    1/500 - 1/1000. Predicted molecular weight: 54 kDa.

    ターゲット情報

    • 機能

      Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

    • 組織特異性

      Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

    • 関連疾患

      Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.

    • 配列類似性

      Contains 1 fork-head DNA-binding domain.

    • 翻訳後修飾

      Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
      Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
      Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.

    • 細胞内局在

      Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • AFX antibody
        • AFX1 antibody
        • Afxh antibody
        • ALL1-fused gene from X chromosome antibody
        • Fork head domain transcription factor AFX1 antibody
        • Forkhead box O4 antibody
        • Forkhead box protein O4 antibody
        • FOXO 4 antibody
        • Foxo4 antibody
        • FOXO4_HUMAN antibody
        • MGC117660 antibody
        • MGC120490 antibody
        • Mixed lineage leukemia, translocated to, 7 antibody
        • MLLT7 antibody
        • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
        • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
        • RGD1561201 antibody
        see all

      画像

      • Western blot - Anti-FOXO4/AFX (phospho T451) antibody (ab79188)
        Western blot - Anti-FOXO4/AFX (phospho T451) antibody (ab79188)
        All lanes : Anti-FOXO4/AFX (phospho T451) antibody (ab79188) at 1/500 dilution

        Lane 1 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins)
        Lane 2 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins) with immunising phosphopeptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 54 kDa
        Observed band size: 54 kDa

      参考文献 (1)

      ab79188 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab79188 は 1 報の論文で使用されています。

      • Salim H  et al. miRNA-214 modulates radiotherapy response of non-small cell lung cancer cells through regulation of p38MAPK, apoptosis and senescence. Br J Cancer 107:1361-73 (2012). PubMed: 22929890

      レビューと Q&A

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      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-FOXO4/AFX (phospho T451) antibody

       Below Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rabbit Tissue lysate - whole (heart)
      Gel Running Conditions
      Non-reduced Denaturing (10)
      Loading amount
      60 µg
      Specification
      heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Feb 09 2012

      Question

      The image is attached.

      Anti-FOXO4 (phospho T451) antibody (ab79188)

      Read More
      Answer

      Thanks for sending me your western blot image. It has been included with your Abreview.

      Please let me know if you need anything further.

      Read More

      Question

      I would like to test this antibody in rabbit, an untested species.

      Read More
      Answer

      I am very pleased to hear you would like to accept our offer and test ab47709, ab51207, and ab79188 inrabbit. This code will give you 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please submit an Abreview for each antibody in rabbitand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com