Anti-FOXO4/AFX (phospho S197) 抗体 (ab47278)
Key features and details
- Rabbit polyclonal to FOXO4/AFX (phospho S197)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXO4/AFX (phospho S197) antibody
FOXO4/AFX 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXO4/AFX (phospho S197) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human FOXO4/AFX aa 150-250 (phospho S197). derived from human FOXO4/AFX around the phosphorylation site of serine 197 (A-A-SP-M-D)
Database link: P98177 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | ||
| WB | (1) | |
| IHC-P |
| 特記事項 |
|---|
IHC: 1/50 - 1/100.
ICC/IF: Use at a concentration of 1-5 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. -
組織特異性
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. -
関連疾患
Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
翻訳後修飾
Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability. -
細胞内局在
Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 4303 Human
- Omim: 300033 Human
- SwissProt: P98177 Human
- Unigene: 584654 Human
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別名
- AFX antibody
- AFX1 antibody
- Afxh antibody
see all
画像
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Western blot - Anti-FOXO4/AFX (phospho S197) antibody (ab47278)All lanes : Anti-FOXO4/AFX (phospho S197) antibody (ab47278) at 1/500 dilution
Lane 1 : Extracts from 293 cells.
Lane 2 : Extracts from 293 cells. Immunizing peptide 1ug/mL
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Peptide - + -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX (phospho S197) antibody (ab47278)This image shows human breast carcinoma stained with ab47781 at 1/50 dilution. Right hand image:tissue was treated with immunogenic peptide, left hand image: untreated.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXO4/AFX (phospho S197) antibody (ab47278)ICC/IF image of ab47278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab47278 は論文での使用が確認できていません。