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リコンビナントRabMAb

Anti-FOXO3A (phospho S253) 抗体 [EPR1951(2)] (ab154786)

Reviews (2)Q&A (1)References (44)
Western blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
  • Dot Blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
  • Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]
    FOXO3A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, Dot blotmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • MCF-7 cell lysate treated with IGF; Human endometrial carcinoma tissue; Human pancreas tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154786の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot
Use at an assay dependent concentration.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.

  • 組織特異性

    Ubiquitous.

  • 関連疾患

    Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.

  • 配列類似性

    Contains 1 fork-head DNA-binding domain.

  • 翻訳後修飾

    In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.

  • 細胞内局在

    Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AF6q21 antibody
      • AF6q21 protein antibody
      • DKFZp781A0677 antibody
      • FKHR2 antibody
      • FKHRL 1 antibody
      • FKHRL1 antibody
      • FKHRL1P2 antibody
      • Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
      • Forkhead box O3 antibody
      • Forkhead box O3A antibody
      • Forkhead box protein O3 antibody
      • Forkhead box protein O3A antibody
      • Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
      • Forkhead homolog (rhabdomyosarcoma) like 1 antibody
      • Forkhead in rhabdomyosarcoma like 1 antibody
      • Forkhead in rhabdomyosarcoma-like 1 antibody
      • FOX O3A antibody
      • FOXO2 antibody
      • foxo3 antibody
      • FOXO3_HUMAN antibody
      • FOXO3A antibody
      • MGC12739 antibody
      • MGC31925 antibody
      see all

    画像

    • Western blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
      Western blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
      All lanes : Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786) at 1/5000 dilution

      Lane 1 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with IGF-1. Whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 71 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)

      Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)

      Immunohistochemical analysis of paraffin-embedded Human normal pancreas tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Dot Blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
      Dot Blot - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)

      Dot Blot analysis of Lane 1: FOXO3A (pS253) phospho peptide and Lane 2: FOXO3A non-phospho peptide with ab154786 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer and concentration. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    • Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
      Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)

    参考文献 (44)

    ab154786 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154786 は 44 報の論文で使用されています。

    • Jin X  et al. miR-506-3p Relieves Neuropathic Pain following Brachial Plexus Avulsion via Mitigating Microglial Activation through Targeting the CCL2-CCR2 Axis. Dev Neurosci 45:37-52 (2023). PubMed: 36470227
    • Wang Y  et al. Proteogenomics of diffuse gliomas reveal molecular subtypes associated with specific therapeutic targets and immune-evasion mechanisms. Nat Commun 14:505 (2023). PubMed: 36720864
    • Yu W  et al. Modulation of SIRT1 expression improves erectile function in aged rats. Asian J Androl 24:666-670 (2022). PubMed: 35229761
    • Liang Z  et al. Wenshen Yangxue decoction promotes follicular development in aged female mice stimulation of the silent information regulator 3/forkhead transcription factor O1 3a pathway. J Tradit Chin Med 42:539-545 (2022). PubMed: 35848970
    • Sun R & Zheng Q AKT/foxo3a signal pathway mediates the protective mechanism of resveratrol on renal interstitial fibrosis and oxidative stress in rats with unilateral ureteral obstruction. Am J Transl Res 14:1788-1795 (2022). PubMed: 35422955
    View all Publications for this product

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (VVEC (vasorum endothelial cells))
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Treatment
    100 uM ATP 0 5 30 min
    Specification
    VVEC (vasorum endothelial cells)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 03 2016

    Western blot abreview for Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (MCF7)
    Specification
    MCF7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 13 2015

    Question


    Inquiry: trying to purchase p-FOXO3a (Ser253)antibody. There are several antibodies for FOXO3a (ab-47285, ab31109, ab154786). They detected different different size of FOXO3a-ser253 protein. Why?

    Read More
    Answer

    Thank you for contacting us.


    The predicted MW of the protein is ˜ 75 kD, based on sequence alone.


    However page 4 of paper attached showed, phosphorylation on serine 253 of FOXO3A/FKHRL1 could migrate the MW to ˜100KD for this protein.


    Read More

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