Anti-FOXO3A (phospho S253) 抗体 (ab47285)
Key features and details
- Rabbit polyclonal to FOXO3A (phospho S253)
- Suitable for: WB, ELISA, IHC-P, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FOXO3A (phospho S253) antibody
FOXO3A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXO3A (phospho S253) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISA, IHC-P, ICC/IF, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human FOXO3A (phospho S253).
Database link: O43524 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47285の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/500 - 1/1000. Detects a band of approximately 67, 105 kDa (predicted molecular weight: 71 kDa).
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
See Abreview. |
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 67, 105 kDa (predicted molecular weight: 71 kDa). |
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ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-Fr
Use at an assay dependent concentration. See Abreview. |
ターゲット情報
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機能
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
組織特異性
Ubiquitous. -
関連疾患
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
翻訳後修飾
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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参照データベース
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 294515 Rat
- Omim: 602681 Human
- SwissProt: O43524 Human
- SwissProt: Q9WVH4 Mouse
- Unigene: 220950 Human
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別名
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
画像
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Western blot - Anti-FOXO3A (phospho S253) antibody (ab47285)All lanes : Anti-FOXO3A (phospho S253) antibody (ab47285) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells untreated
Lane 2 : extracts from NIH/3T3 cells treated with serum
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
Observed band size: 105,67 kDa why is the actual band size different from the predicted?
Serum treatment:-
1. Add fresh basic medium to the vessels and starve the cells for 18-24 hours.
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2. Drain the cultured medium, add growth medium and basic medium containing 10% serum to the vessels.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody (ab47285)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47285 at a 1/50 dilution.Left image : Untreated
Right image : Treated with P-Peptide
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Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A (phospho S253) antibody (ab47285)ICC/IF image of ab47285 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A (phospho S253) antibody (ab47285)This image is courtesy of an anonymous Abreview.ab47285 at 1/200 dilution staining FOXO3A in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permebilized in 0.5% Triton X-100 and then blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/500 dilution. Image show green staining with ab47285 and blue with DAPI.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (58)
ab47285 は 58 報の論文で使用されています。
- Wang K et al. HDAC Inhibitors Alleviate Uric Acid-Induced Vascular Endothelial Cell Injury by Way of the HDAC6/FGF21/PI3K/AKT Pathway. J Cardiovasc Pharmacol 81:150-164 (2023). PubMed: 36607630
- Tang X et al. The combination of Astragalus membranaceus and ligustrazine mitigates cerebral ischemia-reperfusion injury via regulating NR2B-ERK/CREB signaling. Brain Behav 13:e2867 (2023). PubMed: 36585899
- Zhang Y et al. The novel peptide PFAP1 promotes primordial follicle activation by binding to MCM5. FASEB J 37:e22931 (2023). PubMed: 37086099
- Jin L et al. CTLA4-Ig protects tacrolimus-induced oxidative stress via inhibiting the AKT/FOXO3 signaling pathway in rats. Korean J Intern Med 38:393-405 (2023). PubMed: 37157174
- Sasivimolrattana T et al. HPV16E1 downregulation altered the cell characteristics involved in cervical cancer development. Sci Rep 13:18217 (2023). PubMed: 37880374