Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) 抗体 [EPR28359-78]
Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78]
- 20ul selling size
- RabMAb
- Recombinant
- 詳細を見る
Be the first to review this product! Submit a review
|
(1 Publication)
Rabbit Recombinant Monoclonal FOXO1A phospho T24 antibody. Suitable for WB, Dot, Flow Cyt (Intra) and reacts with Transfected cell lysate - Mouse, Human, Mouse, Synthetic peptide samples. Cited in 1 publication.
別名を表示する
Fkhr, Foxo1a, Foxo1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (AB312326)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / NIH/3T3 (mouse embryonic fibroblast) treated with 100nM Calyculin A for 30min (Middle) / Untreated NIH/3T3 (Right) cells labelling FOXO1A (phospho T24) + FOXO3A (phospho T32) + FOXO4/AFX (phospho T28) + FOXO6 (phospho T26) with ab312326 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
- WB
Supplier Data
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (AB312326)
Blocking and diluting buffer and concentration : 5% NFDM/TBST The bands above 100 kDa are unknown. In Western blot, anti-FOXO4 antibody (ab128908) staining at 1/1000 dilution. Exposure time : 59 seconds.
All lanes:
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (ab312326) at 1/1000 dilution
Lane 1:
Jurkat (human t cell leukemia t lymphocyte from peripheral blood) starved overnight, then treated with 50 uM LY294092 for 30min whole cell lysate (untreated membrane) at 20 µg
Lane 2:
Jurkat starved overnight, then treated with 20% serum and 100nM Calycin A for 30min whole cell lysate (untreated membrane) at 20 µg
Lane 3:
Jurkat starved overnight, then treated with 20% serum and 100nM Calycin A for 30min whole cell lysate (phosphatase treated membrane) at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 75-80 kDa
false
Exposure time: 59s
- WB
Supplier Data
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (AB312326)
Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 81 seconds.
All lanes:
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (ab312326) at 1/1000 dilution
Lane 1:
Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg
Lane 2:
NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysate (untreated membrane) at 20 µg
Lane 3:
NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysate (phosphatase treated membrane) at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 75 kDa
false
Exposure time: 81s
- WB
Supplier Data
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (AB312326)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (ab312326) at 1/1000 dilution
Lane 1:
Untreated 293T (human embryonic kidney epithelial cell) transfected with mouse FOXO4/AFX (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 2:
293T transfected with mouse FOXO4/AFX (WT) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg
Lane 3:
Untreated 293T transfected with mouse FOXO6 (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 4:
293T transfected with mouse FOXO6 (WT) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 50 kDa,75 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (AB312326)
Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 6 seconds.
All lanes:
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (ab312326) at 1/1000 dilution
Lane 1:
Untreated 293T (human embryonic kidney epithelial cell) transfected with mouse FOXO3A (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 2:
293T transfected with mouse FOXO3A (WT) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg
Lane 3:
Untreated 293T transfected with mouse FOXO3A (Mutant T32A) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 4:
293T transfected with mouse FOXO3A (Mutant T32A) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 78-140 kDa
false
Exposure time: 6s
- WB
Supplier Data
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (AB312326)
Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-FOXO1A antibody (ab179450) staining at 1/1000 dilution. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 3 minutes.
All lanes:
Western blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (ab312326) at 1/1000 dilution
Lane 1:
Untreated 293T (human embryonic kidney epithelial cell) transfected with mouse FOXO1A (WT) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 2:
293T transfected with mouse FOXO1A (WT) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg
Lane 3:
Untreated 293T transfected with mouse FOXO1A (Mutant T24A) expression vector containi a myc-His-tag® whole cell lysate at 20 µg
Lane 4:
293T transfected with mouse FOXO1A (Mutant T24A) expression vector containi a myc-His-tag®, then treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 78-82 kDa
false
Exposure time: 3min
- Dot
Supplier Data
Dot Blot - Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody [EPR28359-78] (AB312326)
Dot blot analysis of FOXO1A (phospho T24) + FOXO3A (phospho T32) + FOXO4/AFX (phospho T28) + FOXO6 (phospho T26) using ab312326 at 1 : 1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : FOXO1A (phospho T24) peptide 1 Lane 2 : FOXO1A (phospho T24) peptide 2 Lane 3 : FOXO1A (phospho T24) peptide 3 Lane 4 : FOXO1A non-phospho peptide Lane 5 : FOXO3 (phospho T32) peptide Lane 6 : FOXO3 non-phospho peptide Lane 7 : FOXO4 (phospho T28) peptide Lane 8 : FOXO4 non-phospho peptide Lane 9 : FOXO6 (phospho T26) peptide Lane 10 : FOXO6 non-phospho peptide Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST
Reactivity data
製品の詳細
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FOXO proteins influence numerous cellular functions through their role in gene transcription. They are often considered part of larger complexes interacting with various cofactors and other proteins to modulate gene expression. FOXO3A for example plays a role in cellular antioxidant defense energy metabolism and maintenance of cellular homeostasis. Their activity can be modulated by post-translational modifications such as phosphorylation which influences their localization between the cytoplasm and nucleus affecting their function.
Pathways
FOXO proteins are integral components of the insulin-signaling pathway and the PI3K/Akt pathway. Through these pathways they interact closely with proteins such as Akt and PI3K which regulate their activity and subcellular localization. The insulin pathway modulates metabolic processes and glucose homeostasis while the PI3K/Akt pathway is involved in cell survival and growth. Their regulatory functions highlight their adaptive response to environmental and internal cellular signals.
製品プロトコール
- Visit the General protocols
- Visit the Troubleshooting
ターゲットの情報
追加のターゲット
文献 (1)
Recent publications for all applications. Explore the full list and refine your search
Translational andrology and urology 13:1991-2004 PubMed39434735
2024
Applications
Unspecified application
Species
Unspecified reactive species
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com